紫外分光光度法测定珍珠菜中总黄酮含量

Determination of Total Flavones in Lysimachia clethroide Duby by UV Spectrophotometry

目的:建立珍珠菜提取液中总黄酮的含量测定方法。方法:采用紫外分光光度法,通过紫外可见扫描确定最大吸收波长,以芦丁为对照品制定标准曲线,并进行样品溶液稳定性试验和方法回收率与精密度试验。结果:最大吸收波长为405 nm,标准曲线线性范围为4.014-32.112μg/mL,r=0.999 9。样品液经A l3+络合后在0.5-2.5h内稳定性较好,平均加样回收率为102.00%±1.64%,RSD为1.61%,日内、日间精密度RSD小于2%,符合要求。结论:该方法简单方便、准确可靠,可以用来测定珍珠菜中总黄酮的含量。

Objective：To establish the method for in vitro assay of total flavones in Lysimachia clethroide Duby. Methods：UV spectrophotometry method was developed to determine the content of total flavones. Max wavelength was fixed on by violet -visible light scan, and standard curve was established using rutin as a standard compound. Furthermore, stability of samples, recovery of method and precision of instrument were all investigated. Results：The max wavelength was 405 nm, and the linear range of standard curve was 4.014 -32. 112μg/mL, r =0.999 9. Samples were stable in 0.5 -2.5 h after reacted with Al^3＋ The average recovery was 102.00% ± 1.64% ,RSD 1.61%, and all of RSDs of precision were less than 2%. Conclusion：The method is simple,effective and feasible, and can be used to determine the content of total flavones in Lysimahia clethroide Duby.