东北春麦区部分小麦品种资源遗传多样性研究

Detection of Genetic Diversity in Wheat Varieties Based on Gliadin Fingerprinting Patterns in Spring Wheat Belt of the Northeast

利用所收集的东北春麦区不同年代育成的73份小麦品种资源,以小麦醇溶蛋白为生化指纹,采用国际种子检验协会(ISTA)所推荐的小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳方法,对73份小麦品种资源遗传多样性进行了研究和评价。结果表明:不同年代育成小麦品种醇溶蛋白电泳谱带分布频率和多态性差异较大,总的趋势是随着年代的推近,小麦醇溶蛋白多态性比率增加;小麦醇溶蛋白生化指纹聚类分析结果表明,东北春麦区小麦品种资源遗传变异较为丰富。将供试的东北春麦区小麦品种资源分为4个类群,各类群所包含的品种数目差异较大。

A standard acid polyacryamide gel electropheresis （A - PAGE） was used with 73 wheat varieties which were cultivated in different years to evaluate their genetic diversity. As a result, distribution frequencies and polymorphism of gliadin patterns were differentiated among different years and took on a tendency of increase with the years passing. The UPGMA dendogram, based on a similarity matrix by a simple matching coefficient algorithm, delineated the above accessions into 4 major clusters and was in accordance with the available pedigree information. The results demonstrate that the gliadin alleles had abundant variations in the Northeast spring wheat belt.