炙甘草汤对豚鼠心室肌细胞的电生理影响

The Electrophysiology Effect of Zhigancao Decotion on Ventricle Muscle Cell of Rats

目的:研究炙甘草汤对豚鼠心室肌细胞电生理影响。方法:应用常规的玻璃微电极细胞内记录技术,观察炙甘草汤对豚鼠心室肌细胞的动作电位0相幅值(APA),动作电位时程(APD),50%复极化时间(APD50),90%复极化时间(APD90)的影响。结果:(1)用20mg/ml的炙甘草汤灌流后与正常对照组相比细胞电生理变化不明显;(2)用40mg/ml的炙甘草汤灌流后与正常对照组相比动作电位时程(APD)缩短(P<0.05),90%复极化时间(APD90)下降(P<0.05);(3)用80mg/ml的炙甘草汤灌流后与正常对照组相比动作电位时程(APD)明显缩短(P<0.01),50%复极化时间(APD50)、90%复极化时间(APD90)下降(P<0.05)。结论:炙甘草汤能缩短豚鼠心室肌细胞动作电位时程及50%、90%复极化时间。

Objective： To study the electrophysiology effect of Zhigancao Decoction on ventricle muscle cell of rats. Methods：The action potentials were recorded by conventional intracellular microelectrode technique and the amplitude of action potential（APA） and action potentials（APD） were observed. The effect of 50 % and 90 % of duration of action potential（APD50 and APD90 ） on ventricle muscle cell of rats that were perfused with Zhigancao Decoction was observed as well. Results： （1）For the ventricle muscle cell of rats perfused with 20mg/ml Zhigancao Decoction, electrophyslology had no changes compared with control. （2）For the ventricle muscle cell of rats perfused with 40mg/ml Zhigancao Decoction, the date action potentials（APD） ,90% of duration of action potential（APD50） decreasd（P〈0. 05） compared with control. （3） For the ventricle muscle cell of rats perfused with 80mg/ml Zhigancao Decoction, the date action potentials（APD） decreasd greatly （P〈 0. 01） ,50% and 90% of duration of action potential（APD50 and APD90） decreasd（P〈0. 05） compared with control. Conclusion： Zhigancao Decoction makes the ventricle muscle cell of rats＇ date action potentials（APD） and 50 %, 90 % of duration of action potential（APD50 and APD90 ） decrease.