基本消灭日本血吸虫病地区人群循环抗原的检测

DETECTION OF CIRCULATING ANTIGEN IN POPULATION LIVING IN AREAS WITH SCHISTOSOMIASIS BASICALLY ELIMINATED

目的：探讨在基本消灭日本血吸虫病地区检测循环抗原的应用价值。方法：用夹心ＥＬＩＳＡ和斑点ＥＬＩＳＡ检测抗原，用ＩＨＡ检测抗体，抗原阳性者进一步测ＣＯＰＴ。结果：在１１００份样本中，夹心ＥＬＩＳＡ的阳性率为５．１％，斑点ＥＬＩＳＡ的阳性率为６．５％，两者均阳性者４６例，两者均阴性者１０１９例，两者符合率为９６．８％。ＩＨＡ检测抗体的阳性率为２５．３％。有治疗史人群中抗体的阳性率明显高于无治疗史者。结论：抗体的阳性率显著高于抗原，不能反映日本血吸虫感染的实际情况，抗原检测似为可取的诊断方法。尤其是无治疗史人群抗原阳性与ＣＯＰＴ阳性的符合率较高，说明抗原检测的可靠性。

AIM: To evaluate the practical value of circulating antigen detection in Jiaxing County of Zhejiang Province where schistosomiasis had been basically eliminated. METHOD: Sandwich ELISA and dot ELISA were used to detect circulating antigen, IHA was used to detect antibody, the antigen positive cases were further examined with COPT. RESULTS: Of 1 100 serum specimens examined, the positive rate was 5.1% in sandwich ELISA, and 6.5% in dot ELISA. The qualitative results of both methods were positive in 46 and negative in 1 019, the coincidence rate of sandwich ELISA and dot ELISA being 96.8%, and the positive rate of IHA was 25.3%. The positive rate of antibody detection was significantly higher in the population with treatment history than in those without treatment history.  CONCLUSION: Circulating antigen detection is a practicable method for diagnosing schistosomia sis, especially in the population without treatment history. However, there was a higher coincidence rate of COPT with antigen detection than with antibody detection.