接种大剂量约氏疟原虫子孢子对大鼠肝脏超微结构的影响

EFFECT OF INOCULATED SPOROZOITES OF PLASMODIUM YOELII ON THE ULTRASTRUCTURE OF RAT LIVER

目的：研究大剂量接种子孢子悬液后宿主肝脏的早期急性细胞反应。方法：观察一次大剂量接种９×１０６个约氏疟原虫子孢子悬液，３０ｍｉｎ后的肝脏超微结构。结果：枯否氏细胞吞噬活性增加，胞浆内可见许多大小不等的吞噬复合体；肝窦内皮细胞受损；血小板肿胀、粘集，形成微血栓；部分肝实质细胞受损，线粒体空泡化，胞浆内形成灶性溶解性坏死。结论：子孢子接种３０ｍｉｎ肝脏即有急性炎症细胞反应，有部分子孢子被枯否氏细胞杀伤，肝细胞线粒体退变是引起部分原虫退变的原因之一。

AIM: To examine the effect of inoculated sporozoites of Plasmodium yoelii on the early cellular responses of rat liver. METHOD: Ultrastructure of rat liver was observed by transmission electron microscopy 30 minutes after inoculating with nine million sporozoites. RESULTS: Phagocytic activity of Kupffer cells(Kc) was increased. There were a lot of phagolysosomes in the cytoplasm of Kc. Blood platelets were swollen and adhered to Kc or endothelial cells and to form microthrombi. Some hepatic cells were damaged. The mitochondria of hepatic cells were vacuolated and the mitochondrial cristae were dissolved.There were cytolytic focal necrosis in the cytoplasm. CONCLUSION: Acute cellular responses of rat liver occurred 30 minutes after the inoculation of nine million of P. yoelii sporozoites. Some sporozoites were damaged by Kupffer cells. The ultratructural changes of mitochondria of hepatic cells contributed at least partly to the degeneration of exo erythrocytic stage of the parasite.