HCV NS_5和核心抗原在肝细胞癌及癌旁肝组织的表达及意义

ExpressionandsignificanceofHCVNS5andcoreantigeninhepatocellularcarcinomaandthepara-canceroustissuea

采用ＳＰ法（链菌素抗生物素蛋白－过氧化酶连接法）检测４６例肝细胞癌及其３８例癌旁肝组织中ＨＣＶＮＳ５抗原、ＨＣＶ核心抗原和ＨＢｓＡｇ。结果在癌组织中的检出率分别为１５．２％．２１．７％和１９．６％，癌旁组织中的检出率分别为３１．６％、３６．８％和７８．９％；ＮＳ５抗原、核心抗原伴ＨＥｓＡｇ者分别为２１．７％和２３．９％；不伴ＨＢｓＡｇ者分别为８．７％和１０．９％；ＨＢｓＡｇ不伴ＨＣＶ核心抗原、ＮＳ５抗原者分别为４７．８％和５０％。两种ＨＣＶ抗原和ＨＢｓＡｇ均定位于肝细胞和肝癌细胞的胞浆内，其阳性染色细胞呈弥漫、灶状或散在分布。

ExpressionofHCVNS5coreantigenandHBsAgincanceroustissuesof46patientswithhepatocellularcarcinoma(HCC)andpara-canceroustissuesof38patientswerestudiedwithstreptavidinperoxidaseconjugataedmethod(SPmethod).TheresultsshowedthatthedetectionratesofHCVNS5coreantigenandHBsAgwerefoundin15.2%* .21.7%* and19.6%* inHCCgroupand31.6%* .36.8%* and78.9%* inpara-canceroustissuegroup,respectively.NS5coreantigenaasociatedwithHBsAgwerein21.7%* and23.9%* ,thosewithoutHBsAgwere5.7%* and10.9%* ,HBsAgwithoutHCVcoreandNS5antigenswerein47.8%* and50%,respectively.AllthreeantigenswerelocatedinthecytoplasmofhepatocytesandHCCcells,Thecellswithpositiveanigenstainingweredistributeddiffusely,focallyorsporadically.Basingontheabovefindings,itissuggestedthatHCVNS5andcoreantigencanberegardedasadditionalnewdiagnosticmarkersforHCVinfection.HCVandHBVcanbeassociatedwiththedevelopmentofHCC.TheroleoftheHCVNS5coreantigeninthepathogenesisofHCCawaitsfurtherinvestigation.