腹腔神经节毁损对胃扩张兔脊髓c-fos表达的影响

Effects of celiac ganglion damage on expression of c-fos in spinal cord of gastric distension rabbits

目的:观察高强度聚焦超声(High Intensity Focused Ultrasound,HIFU)毁损腹腔神经节(Celiac ganglion,CG)对胃扩张兔脊髓c-fos表达的影响,探讨其治疗疼痛的可能机制。方法:健康成年新西兰大白兔20只,随机分为4组:非扩张非毁损组(nDnB)、扩张非毁损组(DnB)、扩张毁损组(DB)、非扩张毁损组(nDB),每组各5只。在无菌操作下暴露CG,DB、nDB组用HIFU毁损神经节,nDnB、DnB组假手术,术后7天,均在麻醉下安置胃扩张装置。安置后7天,nDB、nDnB组在麻醉下灌注固定取胸段6～8(T6～8)脊髓,DB和DnB组采用反复梯度扩张胃1h后,灌注固定取脊髓。用免疫组织化学方法检测脊髓前角和后角Fos样免疫反应阳性神经元(Fos-1ike immunoreactivity neuron,FLIN)计数的变化。结果:各组FLIN比较,毁损腹腔神经节和(或)扩张胃均不能使脊髓后角及前角FLIN明显地增加(P>0.05)。结论:HIFU毁损CG对胃扩张兔脊髓c-fos表达无明显影响,这与其它内脏神经毁损对伤害性刺激引起的脊髓c-fos表达的影响不同,可能是因为对胃伤害性信息的介导存在特殊的中枢机制。

Objective：To observe the influence of high intensity focused ultrasound （HIFU）damaging the celiac ganglion （CG） of rabbits on expression of c-fos in spinal cord following gastric distension, and study the potential mechanism in treating pain. Mothods：20 healthy and grown New Zealand rabbits were randomly divided into four groups： not distend damage （nDB）, distend damage（DB）,not distend not damage（nDnB）,distend not damage（DnB）,with 5 in each group. At first, the CG was exposed under aseptic manipulation, then group nDB and DB were damaged by HIFU, group nDnB and DnB not damaged. 7 days later, they all were installed with the device of gastric distension under anaesthesia. In group nDB and nDnB tissue were directly fixed through infusing 4% parafomaldehyde under deep anaesthesia, then spinal cord of thoracic 6-8（T6-8）extracted. In group DB and DnB stomach was distended step by step. Repeat distension for 1h, the spinal cord were extracted in the way similar to that the above two groups. The number of fos-like immunoractivity neurons （FLIN） in spinal cord was displayed using immunohistochemistry technique of Strept Avidin Biotin Complex （SABC）. Results：Damaged CG and gastric distension as well as damaged CG combined with gastric distension could not induce the FLIN in dorsal horn as well as the anterior gray horn of thoracic slices to significantly increase（P〉0.05）. Conclusion：HIFU damaging the celiac plexus（CG） has no influence on expression of c-fos in spinal cord of gastric distension rabbits. This is different from the expression of c-fos in spinal cord caused by other visceral nerves being damage of nociceptive stimulus, which might relate to the special central mechanism of modulating nociceptive signal for stomach.