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人宫颈癌Ca Ski细胞IL-24基因的克隆鉴定与真核表达 被引量：1

Molecular cloning,identification and eukaryotic expression of IL-24 gene in Ca Ski cells
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摘要 目的:克隆人IL-24基因并构建真核表达载体,转染Ca Ski细胞进行真核表达.方法:利用RT-PCR技术扩增出Ca Ski细胞中的IL-24基因.将IL-24基因克隆入pcDNA3.1(+)中,构建真核表达质粒pcDNA3.1(+)-hIL-24.将重组质粒转染入CaSki细胞,利用Western blot检测hIL-24的表达.结果:PCR及测序结果均证实成功克隆了人IL-24基因,构建了真核表达质粒pcDNA3.1(+)-hIL-24,该质粒被转染入Ca Ski细胞中能表达出hIL-24蛋白.结论:成功构建了hIL-24基因的真核表达载体. AIM： To clone human IL-24 （ hIL-24 ） gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. METHODS： hIL-24 gene was acquired by RT-PCR from the total RNA of Ca Ski cell and cloned into plasmid pcDNA3. 1 （ ＋ ） to generate the eukaryotic expression vector named pcDNA3. 1 （ ＋ ）-hIL-24. pcDNA3. 1 （ ＋ ）-hIL-24 was transfected into Ca Ski cells to express hIL-24 protein. RESULTS： PCR and sequencing revealed that hIL-24 gene was successfully cloned into plasmid pcDNA3.1 （ ＋ ） and its eukaryotic expression vector was constructed. The vector was able to express hIL-24 protein after transfected into Ca Ski cells. CONCLUSION： The hIL-24 eukaryotic expression vector has been successfully constructed.
作者 石华 成海恩 易发平 杨俊霞 张溢 马永平 宋方洲
机构地区 重庆医科大学生物化学与分子生物学教研室重庆市生物化学与分子药理学重点实验室临床检验诊断学教育部重点实验室重庆医科大学基础医学研究所
出处 《第四军医大学学报》 北大核心 2007年第5期469-471,共3页 Journal of the Fourth Military Medical University
基金 重庆市教委重点项目(渝教科[2001]12号)
关键词 白细胞介素24 克隆 真核表达 CA Ski细胞 IL-24 clone eukaryotic expression Ca Ski cell
分类号 Q78 [生物学—分子生物学]
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参考文献5

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二级参考文献10

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第四军医大学学报
2007年 第5期

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