摘要
目的分析北方一寻常型银屑病家系的发病与HLA-A、B、DRB1等位基因的相关性。方法采用序列特异性寡核苷酸探针-聚合酶链反应(PCR-SSO)方法检测一北方家系16人的HLA-A、B、DRB1等位基因。结果家系中共有6人患寻常型银屑病,其中4个早发寻常型银屑病患者(Ⅱ-2、Ⅱ-4、Ⅲ-1、Ⅲ-5)发病均在30岁以前,B位点均为B*13、DRB1位点均为DRB1*07;家系中有6人携带单倍型11-15-15,其中4人发病(Ⅰ-2、Ⅱ-2、Ⅱ-4、Ⅲ-1),年龄稍小的2人未发病(Ⅲ-3、Ⅲ-4)。结论此家系中寻常型银屑病的发病涉及多个易感位点,携带B*13-DRB1*07基因的早发病者可能与对遗传因素和/或环境因素敏感性增加有关;易感单倍型携带者的发病受环境因素和/或其他易感因素的影响。
Objective members with psoriasis investigate correlation of HLA class Ⅰ and Ⅱ alleles in a Northern Chinese Family vulgaris. Methods The polymerase chain reaction amplification with sequence-specific oligonucleotide probe （PCR-SSO） method was used to analyze the alleles of HLA-A ,B,DRB1 in all the family membets. Results HLA-B ＊ 13-DRB1 ＊ 07 was identified as a high-risk haplotype in four early-onset psoriatic membets （ Ⅱ-2, Ⅱ-4, Ⅲ-1, Ⅲ-5） （age of onset 〈40y） ; while HLA-A ＊ 11-B ＊ 15-DRB1 ＊ 15 was identified as a susceptible haplotype in the six members in which there were four patients （ Ⅰ -2, Ⅱ-2, Ⅱ-4,Ⅲ-1 ）. Conclusion A positive association of the haplotype 1-13-7 with the early -onset psoriasis vulgaris was identified. It may be due to increase individual＇ s sensitivity to genetic factors as well as environmental factors. The penetrance of the haplotype susceptible to psoriasis was affected by many factors.
出处
《基础医学与临床》
CSCD
北大核心
2007年第3期267-270,共4页
Basic and Clinical Medicine
基金
北京市自然科学基金(7022034)
