阿托伐他汀抑制THP-1经PMA诱导后变成巨噬细胞ATP结合盒转运子A1mRNA和蛋白的表达

Atorvastatin inhibits the expression of ATP-binding cassette transporter A1 mRNA and protein in THP-1 macrophages induced by PMA

目的探讨阿托伐他汀对THP-1巨噬细胞ATP结合盒转运子A1(ABCA1)mRNA和蛋白及LXRαmRNA表达的影响。方法体外培养的人THP-1单核细胞,经氟波酯(PMA)诱导48 h,使形成巨噬细胞,将分化良好的巨噬细胞分别加入含不同浓度或不含阿托伐他汀的无血清RPMI 1640培养基,培养24 h或48 h,收集细胞。用多聚酶链反应(RT-PCR)和免疫组化法检测THP-1巨噬细胞ABCA1 mRNA和蛋白及LXRαmRNA的表达。结果含阿托伐他汀(10μmol/L)的培养基体外培养人THP-1巨噬细胞24 h,ABCA1(相对表达量IA值1.21,对照组1.48)和LXRαmRNA(A值0.87,对照组1.12)表达比对照组明显减少(P<0.05),延长培养时间,ABCA1和LXRαmRNA表达有进一步减少趋势。结论阿托伐他汀抑制体外培养的人THP-1巨噬细胞ABCA1 mRNA和蛋白及LXRαmRNA的表达。

Objective To investigate the effect of atorvastatin on expression of ABCA1 （ mRNA, protein） and LXRα mRNA in THP-1 macrophages induced by PMA. Methods Cultured THP-1 cells were induced to differentiate into maerophages by PMA for 48 hours. The macrophages were incubated with atorvastatin in different concentions for 24 or 48 hours. We determined the changes of ABCAI mRNA, protein and LXRα mRNA by reverse trancriptase polymerase chain reaction （RT-PCR） and immuno- histochemistry. Results The expression level of ABCAI mRNA （ratio of relative expression 1.21 vs 1.48） and protein as well as LXRα mRNA（0. 87 vs1. 12） were decreased in THP-lmacrophages when cultured with atorvastatin（ 10 μmol/L） for 24 h. Conclusion Atorvastatin inhibits the expression of ABCA1 mRNA and protein as well as LXRα mRNA of THP-1 macrophages in vitro.