血管紧张素Ⅱ及其受体拮抗剂对肝星状细胞Ⅰ型胶原合成的影响

Effects of AngiotensinⅡ and Its Receptor Antagonist on CollagenⅠSynthesis of Hepatic Stellate Cells

目的观察血管紧张素Ⅱ(AngⅡ)及其受体拮抗剂(AT1RA)对体外培养的肝星状细胞Ⅰ型胶原蛋白合成及其mRNA表达的影响。方法采用HSC-T6肝星状细胞系作为活化的肝星状细胞的研究模型。将培养的肝星状细胞分为对照组、AngⅡ组、AT1RA组和AngⅡ+AT1RA组。采用ELISA法检测细胞培养上清液中Ⅰ型胶原蛋白的含量。RT-PCR法检测肝星状细胞中Ⅰ型胶原mRNA的表达。结果细胞培养上清液中Ⅰ型胶原蛋白含量及肝星状细胞Ⅰ型胶原mRNA的表达水平,AngⅡ组均高于对照组,差异有统计学意义(P<0.05),AT1RA组与AngⅡ+AT1RA组均低于对照组,差异有统计学意义(P<0.05)。结论AngⅡ能够促进肝星状细胞Ⅰ型胶原蛋白的合成及其mRNA的表达,而AT1RA能够明显抑制这一作用。

Objective To investigate the effects of angiotensin Ⅱ （ Ang Ⅱ ） and angiotensin Ⅱ type 1 receptor antagonist （AT1RA） on the collagen Ⅰ synthesis and collagen Ⅰ mRNA expression of cultured hepatic stellate cells （HSCs） in vitro. Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cells. Cultured HSCs were randomized into control group, Ang Ⅱ group, AT1RA group and Ang Ⅱ ＋ AT1RA group. Cell culture medium was used to detect the collagen Ⅰ level with ELISA method. HSCs were harvested to measure the collagen Ⅰ mRNA expression with RT-PCR. Results The collagen Ⅰ level and collagen Ⅰ mRNA expression level of Ang Ⅱ group was higher than control group（ P 〈 0.05 ）, AT1RA group and Ang Ⅱ ＋ AT1RA group was lower than control group （ P 〈 0. 05 ）. Condusion Angiotensin Ⅱ can increase the collagen Ⅰ synthesis and collagen Ⅰ mRNA expression of hepatic stellate cells, while all these effects are inhibited by angiotensin Ⅱ type 1 receptor antagonist.