黑色素瘤抗原-4基因在结肠腺癌中表达的检测

Test for Expression of Melanoma Antigen-4 Gene in Human Colonic Adenocarcinoma Tissue

目的检测黑色素瘤抗原-4(MAGE-4)基因在结肠腺癌中的表达情况。方法采用RT-PCR方法检测44份结肠腺癌组织及15份癌旁正常组织标本中MAGE-4基因的表达。结果44份结肠腺癌标本中,MAGE-4基因的阳性表达率为38.64%,而在15份癌旁正常组织中均不表达,两组比较差异有显著意义。对阳性标本的RT-PCR扩增产物中目的基因片段进行DNA测序,证实为MAGE-4全长编码基因。结论MAGE-4基因在结肠腺癌组织中呈特异性表达,提示该基因可用于结肠癌疫苗的开发。

Objective To test the expression of melanoma antigen-4 （MAGE-4） gene in human colonic adenocarcinoma tissue. Methods The expression of MAGE-4 gene in 44 human colonic adenocarcinoma tissue specimens and 15 normal colon tissue specimens were tested by RT-PCR. Results The positive rate of MAGE-4 was 38. 64% in 44 human colonic adenocarcinoma tissue specimens. However, no expression of MAGE-4 gene was detected in 15 normal colon tissue specimens. The RT-PCR product of specimens positive for MAGE-4 was sequenced and proved as the full length of MAGE-4 gene. Conclusion Specific expression of MAGE-4 gone was proved in human colonic adenocarcinoma tissue,which indicated that MAGE-4 gene might be used for the development of colonic carcinoma vaccine.