摘要
目的观察重组HBcAg与HBsAg混合抗原免疫BALB/c小鼠后体液免疫与细胞免疫的效果。方法配制含Al(OH)3佐剂与不含佐剂的混合抗原,分别免疫BALB/c小鼠,采用ELISA法测定抗体滴度和细胞因子水平;采用流式细胞仪分析T细胞亚群。结果HBcAg与HBsAg混合抗原免疫小鼠产生的IFN-γ量明显高于二者单独免疫,CD3+和CD4+水平显著增高;含Al(OH)3佐剂的混合抗原CD8+水平明显高于阴性对照组;混合抗原免疫小鼠血清产生的抗-HBs抗体与2倍剂量的HBsAg单独免疫相比,差异无显著意义。含Al(OH)3佐剂与不含佐剂的疫苗免疫效果差异无显著意义。结论重组HBcAg与HBsAg混合抗原表现出良好的体液免疫与细胞免疫效果,为治疗性乙肝疫苗的开发提供了依据。
Objective To observe the humoral and cellular immune effects of recombinant HBcAg and HBsAg mixed antigen in mice. Methods Immunize BALB/c mice with the prepared adjuvant-free and aluminium hydroxide adjuvant-containing recombinant HBeAg and HBsAg mixed antigens respectively. Determine the serum antibody and eytokine levels of the mice by ELISA, and analyze the T cell subgroups by flow cytometry. Results The IFN-γ, CD3^＋ and CD4^＋ levels of mice immunized with mixed antigen were sig- nitlcantly higher than those with HBcAg or HBsAg only. The CD8^＋ level of mice immunized with aluminium hydroxide adjuvant-containing mixed antigen was significantly higher than that with physiological saline as control. The serum anti-HBs level of mice immunized with mixed antigen showed no significant difference from that with HBsAg at double dosage. No significant difference was observed between the immune effects of adjuvant-free and aluminium hydroxide adjuvant-containing antigens. Conclusion Recombinant HBcAg and HBsAg mixed antigen showed good humoral and cellular immune effects,which provided a basis for the development of therapeutic hepatitis B vaccine.
出处
《中国生物制品学杂志》
CAS
CSCD
2007年第3期198-200,共3页
Chinese Journal of Biologicals
