摘要
目的:建立快速测定人血浆中复方氨酚帕马溴含量的高效液相色谱法。方法:在盐酸酸性条件下用乙酸乙酯对血样进行提取,采用Diamonsil ^(TM)C_(18)柱,以茶碱为内标,同时测定血浆中对乙酰氨基酚和帕马溴的浓度。流动相:0.2%三氟乙酸水溶液:乙腈=87:13(V/V);检测波长:0.00~8.00 min为249 nm,8.01~13.00 min为278 nm。结果:对乙酰氨基酚和帕马溴的保留时间分别为5.9 min和11.3 min, 2药的线性范围都是0.02~20 mg·L^(-1),提取回收率大于60%(n=5),方法回收率大于90%(n=5),日内、日间RSD小于7.5%(n=5)。结论:本试验方法简便快速、灵敏准确,适用于复方氨酚帕马溴制剂药动学、生物等效性研究。
AIM: To establish a in compound acetaminophen and from plasma with ethyl acetate in pamab rapid HPLC method rom tablets in human for determination of acetaminophen and pamabrom plasma. METHODS: The samples were extracted hydrochloric acid surroundings. Chromatograph was carried out on a Diamon-sil^TM C18 column, using theophylline as internal standard, and the concentrations of acetaminophen and pamabrom were determined simultaneously. The mobile phase was 0.2 % trifluoroacetic acid-acetonitrile (87 : 13, V/V), the detection wavelength was set as follows: 0.00 - 8.00 min: 249 nm, 8.01 - 13.00 min: 278 nm. RE- SULTS: The retention time of acetaminophen and pamabrom were approximately 5.9 min and 11.3 min, respectively. A good linearity was shown in the concentration range 0.02 - 20 mg·L^-1 for both constituents. The extraction recovery was more than 60 % (n = 5) and the method recovery was more than 70 % (n = 5). The interday or intraday precision was less than 7.5 % in replicate analysis (n = 5). CONCLUSION: This HPLC method is simple, quick, sensitive and accurate. It is suitable for clinical pharmacokinetic or bioequivalence study of compound acetaminophen and pamabrom tablets.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2007年第3期187-190,共4页
Chinese Journal of New Drugs and Clinical Remedies
关键词
对乙酰氨基酚
帕马溴
色谱法
高压液相
复方氨酚帕马漠
血药浓度
acetaminophen
pamabrom
chromatography, high pressure liquid
compound acetaminophen and pamabrom
plasma drug concentration