HPLC测定枳壳饮片中柚皮苷的含量

Determination of Naringin in Fructus Aurantii Praeparata by HPLC

目的：建立HPLC测定枳壳饮片中柚皮苷定量分析方法。方法：色谱柱：Lichrospher ODS C18柱（4．6mm×250mm，5μm），流动相：甲醇-水（40：60），检测波长：283nm，流速：1．0mL·min^-1，柱温为室温。结果：在上述色谱条件下，柚皮苷对照品进样量在16．7—100．2μg·mL。内与其峰面积呈良好的线性关系，r=0．9999。对照品和样品精密度试验的RSD分别为0．38％和0．65％（n=5）。对照品和样品稳定性试验的RSD分别为0．28％和0．37％（n=5）。重复性试验RSD为0．31％（n=5）。平均回收率为99．08％，RSD为1．37％（n=5）。结论：该测定方法简便易行，结果准确，重复性好，可用于测定枳壳饮片中柚皮苷的含量。

Objective： To establish a method for the content determination of naringin in fructus aurantii praeparata. Methods： Chromatographic column： Lichrospher ODS CIS （4. 6mm ×250mm, 5μm） was used, the mobile phase was Methanol - water （40：60）, the flow rate was 1.0mL·min^-1, the wavelength of detector was 283nm, the column temperature was room temperature. Results： Under the chromatographic conditions, in the range of 16. 7 - 100. 2μg·mL^-1, the injection of naringin and its peak area were in good line （r =0. 999 9） ; the RSD of control substance and testing substance in precision test were 0. 38% and 0. 65% （n =5） , the RSD of control substance and testing substance in stability test were 0. 28% and 0. 37% （n =5）, the RSD of reproducibility test was 0. 31% （n =5） ; the average recovery was 99.08% and its RSD was 1.37% （ n =5） . Conclusion： The method was accurate and with good reproducibility, which can be used as a quantitative analysis method for naringin in fructus aurantii praeparata.