中药龟板活性部位GC-MS生物活性指纹图谱和应用

GC-MS fingerprint of effective components extracted from plastrum testudinis and its application

目的探求调控骨髓间质干细胞中药的共性。方法硅胶柱层析龟板活性部位浸膏,用石油醚-乙酸乙酯作洗脱剂,梯度洗脱,得到16个样品;采用MTT法研究了它们对rMSCs的增殖调控作用,根据总离子图的相似保留时间和相应峰面积,获得促进rMSCs的增殖的指纹图;同样根据它们的总离子图的相似保留时间和相应峰面积也获得了抑制rMSCs增殖的指纹图;用中药龟板提取物有效部位指纹图谱预测中药汤剂“四物汤”和“栽培红厚壳果仁”共7个样品。结果活性实验结果显示Ts-2、Ts-3、Ts-11、Ts-12、Ts-16能促进rMSCs的增殖作用(P<0.05);Ts-4对rMSCs具有明显抑制作用,其他样品对rMSCs的增殖作用不显著(P>0.05),不具统计学意义;龟板活性部位指纹图谱预测其他中药活性正确率为86%。结论这种以生物活性为基础的指纹图谱具有科学性,为寻找相似活性的中药提供新思路。

Objective To determine the similarities of Chinese traditional herbs for rat mesenchymal stem cells rMSCs. Methods Ointment extracted from plastrum testudinis was separated by silica gel column chromatography. Petroleum Ether-ethyl acetate was used as the eluent, and 16 components were obtained. To establish the fingerprint of volatile oil of plastrum testudinis, GC-MS was carried out. In order to screen the effective components, MTT assay on the proliferation of mesenchymal stem cell （MSC） was also performed. Extracts of SWT and extracts of the seeds from the wild Calophyllum with fingerprint of plastrum testudinis were compared. Results Cell viability in component 2, 3, 11, 12, 16 was increased compared with that of the control group （P〈0. 05）, while cell viability in component 4 was decreased. Cell viability in the other components was similar to that of the control group （P〉0.05）. The validity of comparison of other Chinese traditional herbs with fingerprint of plastrum testudinis was 86%. Conclusion The fingerprint based on the activity is scientific, and this method provides a new way to study the herbs.