上皮性卵巢癌中自噬基因Beclin 1的表达及其调控相关信号传导途径的研究

Expression and Involved Signal Transduction Pathway of Autophagy Gene Beclin 1 in Epithelial Ovarian Cancer

目的研究自噬基因Begin1及其调控相关信号传导途径磷酸肌醇-3激酶／蛋白激酶B（P13K／PKB）与上皮性卵巢癌发生发展之间的关系。方法用免疫组化的方法对25例正常卵巢组织、25例良性上皮性卵巢肿瘤、19例交界性上皮性卵巢肿瘤及69例上皮性卵巢癌组织进行Beclin1、ClassⅠP13K（p110α）、ClassⅡP13K（hvps34）及其下游的磷酸化PKB（p-PKB）的表达水平进行检测。结果Beclin1、hvps34在正常卵巢组织与良性卵巢肿瘤组织中表达较高，在交界性卵巢肿瘤组织中开始下降，在上皮性卵巢癌中的表达最低（P＜0．05）；p110a、P-PKB在交界性卵巢肿瘤组织中开始升高，在上皮性卵巢癌中的表达高于其它3组（P＜0．05）。卵巢癌Ⅰ～Ⅱ期、高中分化、淋巴结无转移的卵巢肿瘤组织中Beclin1的表达分别高于Ⅲ～Ⅳ期、低分化、淋巴结有转移者（P＜0．05），而p110α及P—PKB的表达则分别降低（P＜0．05）。结论自噬基因Beclin1在上皮性卵巢癌中的表达下词，对自噬起调控作用的P13K／PKB信号通路中的成分p110α、hvps34、P—PKB存在表达异常，可能与上皮性卵巢癌的发生、发展及临床预后有关。

Objective To investigate the relationship between the autophagy gene Beclin 1 involving the PI3K/PKB signaling pathway and the occurrence, development of epithelial ovarian carcinoma. Methods The expression of Beclin 1, Class Ⅰ PI3K （p110α）, Class Ⅲ PI3K （hvps34） or p-PKB was, by immunohistochemistry, detected respectively in 25 normal ovarian tissues, 25 benign neoplasia tissues, 19 borderline tissues, and 69 epithelial ovarian carcinoma tissues. Results The higher expressions of Beclin 1 and hvps34 were found in normal and benign ovarian neoplasia tissues ; the expressions were reduced in the borderline lesion tissue ; and the lowest level of expressions could he detected in the ovarian carcinoma tissue （P〈0. 05）. The expressions of p110α and p-PKB increased slightly in borderline ovarian tissue, and were higher in the epithelial ovarian carcinoma tissue than other three groups （P〈0. 05）. Beclin Ⅰ expressions in the epithelial ovarian cancer tissues with stage Ⅰ - Ⅱ , high-middle grade differentiation and negative lymph node metastasis were higher than those with stage Ⅲ-Ⅳ , low grade differentiation and positive lymph node metastasis, but the expressions of p110α and p-PKB in were lower （P〈0. 05）. Conclusion Beclin 1 expression is down-regulated in epithelial ovarian cancer tissue while the p110α, hvps34 and p-PKB are having the abnormal expressions on PI3K/PKB signaling pathway, which may be correlated with the occurrence and development of epithelial ovarian carcinoma.