静磁场对人牙龈成纤维细胞超氧化物歧化酶活性的影响

Effects of Static Magnetic Field on the Activity of Superoxide Dismutase of Human Gingival Fibroblasts

目的研究不同磁路设计的磁性附着体所产生静磁场对人牙龈成纤维细胞酶学的作用。方法使用自主设计的磁场加载系统,产生不同强度的静磁场,对体外培养的人牙龈成纤维细胞进行不同时间的磁场加载。通过与对照组的比较,探讨磁场对该类细胞超氧化物歧化酶活性的影响。结果改变加载强度(120mT、10mT、0mT)或加载时间(1、3、5个加载周期),静磁场对人牙龈成纤维细胞超氧化物歧化酶活性的影响差异均无统计学意义。结论本试验条件下,开放及闭合磁路系统磁性附着体所产生静磁场,对牙龈成纤维细胞超氧化物歧化酶活性没有影响。

Objective To investigate the effects of static magnetic fields （SMF） generated by the opencircuit and closed-circuit dental magnetic attachments on the enzymology of human gingival fibroblasts. Methods In this study, a cellular SMF exposure system developed by our department of prosthetic dentistry was used to impose the static magnetic force on the cultured human gingival fibroblasts in vitro. The activity of superoxide dismutase in those fibroblasts of the test group and that of the control group were measured and compared. Results No significant difference （P〉0. 05） in superoxide dismutase activity was found between the groups when they were loaded with different intensity of magnetic fields or for different period of time. Conclusion Under the conditions in this experiment, the static magnetic fields of the open-circuit magnetic attachments, and those of the closed-circuit ones as well, have no biological effects on the superoxide dismutase of human gingival fibroblasts.