Aurora A表达与人前列腺癌细胞对紫杉醇敏感性的相关性研究

Study on the Relationship between Aurora A Expression in Cancer Cell and Treatment Efficacy of Taxol for Prostate Cancer

目的探讨前列腺癌细胞中Aurora A表达与细胞对紫杉醇敏感性的相关性,明确Aurora A表达是否影响紫杉醇对前列腺癌的治疗效果。方法以激素非依赖性前列腺癌细胞Du145为研究对象,通过转染Aurora A全长基因得到稳定高表达Aurora A的细胞系Du145-Aurora A,细胞用不同浓度紫杉醇处理,用MTT细胞增殖实验检测细胞生长抑制率,用流式细胞仪分析细胞凋亡情况,比较Aurora A表达上调后细胞生长抑制率和细胞凋亡率的变化。同时,用核酶技术使细胞Aurora A表达下调,检测Aurora A表达下调后细胞生长抑制率和细胞凋亡率的变化。结果Aurora A表达上调使紫杉醇对细胞的生长抑制率和细胞凋亡率均降低(P<0.01),而Aurora A表达下调使紫杉醇对细胞的生长抑制率和细胞凋亡率均增高(P<0.01)。结论人前列腺癌细胞中Aurora A表达与细胞对紫杉醇敏感性具有相关性,抑制Aurora A可望提高紫杉醇对前列腺癌的治疗效果。

Objective To explore the relationship between Aurora A expression and cell sensitivity to taxol, and elucidate the effect of Aurora A on the treatment efficacy of taxol for prostate cancer. Methods A cell clone highly expressing Aurora A was constructed through transfected with full length Aurora A gene, and then termed as Du145-Aurora A. Cells were treated with different dose taxols. The growth inhibition rates of cells were detected by MTT assay, and the cell apoptosis rates were detected through flow cytometry. The growth inhibition and apoptosis rates of Du145-Aurora A and Du145 cells were compared for Aurora A up-regulated expression. Simultaneously, cells Du145-Aurora A were treated with DNAzymes for down-regulating the Aurora A expression, and then the variations of cell growth inhibited rate and apoptosis rate were detected. Results Upregulation of Aurora A expression reduced the cell inhibition and apoptosis rates when treated by taxol, but the down-regulation of Aurora A expression after DNAzymes applied increased the cell inhibition and apoptosis rates when treated by taxol. Conclusion There is a positive relationship between Aurora A expression and treatment efficacy of taxol for prostate cancer, and inhibiting Aurora A expression will enhance the treatment efficacy of taxol for prostate cancer.