转染节律基因mPeriod2对小鼠正常细胞放射损伤的影响

Effects of circadian gene mPeriod2 transfected on radiation injury of normal cells of mice

目的评价mPeriod2(mPer2)基因转染后过表达对小鼠肺成纤维细胞NIH3T3放射损伤的影响。方法采用脂质体介导法将mPer2基因转染入NIH3T3细胞中,以空质粒pcDNA3.1和空白组为对照,采用流式细胞术检测mPer2基因在NIH3T3细胞中的表达,经过^(60)Co-γ射线放射处理后,用流式细胞术检测细胞凋亡和细胞周期分布,采用平板克隆形成试验检测细胞增殖情况。结果射线照射后,与pcDNA3.1空质粒组和空白对照组相比,pcDNA3.1-mPer2转染组凋亡率下降,S期细胞所占比例增高,克隆形成率明显增高。结论节律基因mPer2过表达会使γ射线照射后的NIH3T3细胞凋亡下降,增殖加快,减弱了细胞的放射损伤。

Objective To study the effects of circadian gene rnPeriod2 （roPer2） over-expression on radiation injury of NIH3T3 cells. Methods Lipofectarnine -- mediated gene transfection method was used to transfect rnPer2 gene into NIH3T3 cells. The expression of roper2 gene was detected by flow cytometry. After ^60Co-γ ray radiation, the cell apoptosis and distribution in cell cycle were detected by flow cytometry, and colony formation assay was used to detect the cell proliferation. Results As compared with the cells transfected with pcDNA3.1 and the cells without transfection, the cells transfected with pcDNA3. 1-mPer2 had the lower rate of apoptosis, the higher percentage of cells at S stage and the high- er colony-forming efficiency. Conclusion After irradiation, over- expression of rnPer2 in NIH3T3 cells results in reduced apoptosis and enhanced cell proliferation, suggesting that overexpressed rnPer2 may attenuate radiation injury of the cells.