摘要
用5′锚定PCR技术分离出32个枇杷特异SSR位点,对其中适合设计引物的28个位点设计了相应的引物,并分别与对应的5′锚定的简并SSR引物配对,应用于5个枇杷品种的基因组PCR扩增。结果表明,27条引物在5个受试枇杷品种中有扩增产物,其中24条为可用引物,另外3条引物因为扩增产物不符合SSR标记的特征不能用作SSR标记的引物。
Twenty-eight SSR primers were designed according to 32 SSR specific loci of Eriobotrya japonica isolated by 5' anchor primer(s). These SSR primers with their corresponding 5 'anchor degenerated primer(s) were applied in amplification of five cuhivar genomes of Eriobotrya japonica. Twenty-seven primer pairs could efficiently amplify the genomes. Based on the characteristics of SSR products ,24 primer pairs could be used in further Eriobotrya japonica genome research.
出处
《江苏农业学报》
CSCD
北大核心
2007年第1期50-53,共4页
Jiangsu Journal of Agricultural Sciences
基金
上海市科技兴农重点攻关项目(沪农科工字2005-5-5)