利用5′锚定PCR技术分离枇杷微卫星标记

Isolation of Microsatellite Markers in Eriobotrya japonica Using 5′ Anchored PCR

用5′锚定PCR技术分离出32个枇杷特异SSR位点,对其中适合设计引物的28个位点设计了相应的引物,并分别与对应的5′锚定的简并SSR引物配对,应用于5个枇杷品种的基因组PCR扩增。结果表明,27条引物在5个受试枇杷品种中有扩增产物,其中24条为可用引物,另外3条引物因为扩增产物不符合SSR标记的特征不能用作SSR标记的引物。

Twenty-eight SSR primers were designed according to 32 SSR specific loci of Eriobotrya japonica isolated by 5＇ anchor primer（s）. These SSR primers with their corresponding 5 ＇anchor degenerated primer（s） were applied in amplification of five cuhivar genomes of Eriobotrya japonica. Twenty-seven primer pairs could efficiently amplify the genomes. Based on the characteristics of SSR products ,24 primer pairs could be used in further Eriobotrya japonica genome research.