高效毛细管电泳法测定苦参碱脂质体中苦参碱的含量

HPCE Determination of Matrine in Matrine Liposome

目的：建立高效毛细管电泳法测定苦参碱脂质体中苦参碱的含量。方法：毛细管（150cm×75μm），0.25mol／L磷酸缓冲液（pH6．8），高压进样5s，分离电压20kV，温度为25℃，检测波长为206nm.结果：苦参碱在0.3～6.02mg／ml浓度范围内线性关系良好（r=0．9995），苦参碱的平均回收率为97．3％。结论：本方法准确、简单、快捷，适用于苦参碱脂质体的质量控制。

Objective： To establish an HPCE method for the determination of matrine in Matrine Liposome. Mehtods： HPCE was used. The best condition was achieved with a fused silica capillary tube（150 cm ×75 μm）, 0. 25 moL/L phosphate buffer（pH 6. 8）, at constant voltage of 20 kV and temperature at 25℃. The UV detection wavelength was 206 nm. Results： The calibration curve was linear in the range of 0. 3 -6. 025 mg/ml for matrine（0. 9995）. The average recovery of matrine was 97.3%. Conclusion： This methods is simple, quick, accurate and suitable for the quality control for Matrine Liposome.