阿茨海默氏病与肿瘤坏死因子水平及肿瘤坏死因子TNF-α-308G/A基因多态性的关系

Study on the Plasma TNF Level and TNF-α-308G/Apolymorphism in Patients with Alzheimer's Disease

目的探讨肿瘤坏死因子A-308基因多态性与阿茨海默氏病的关系。方法用放免法测定TNF水平,运用多聚酶链反应技术检测66例阿茨海默氏病及143例正常人肿瘤坏死因子A-308基因多态性。结果AD组肿瘤坏死因子A-308TNF-α1/1、TNF-α1/2、TNF-α2/2表型频率分别为0.8636、0.1212、0.0152,对照组分别为0.8881、0.1049、0.070;AD组TNF-α1、TNF-α2基因频率分别为0.9242、0.0758;对照组分别为0.9021、0.0979,肿瘤坏死因子A-308各种基因型频率在患者组与正常对照组之间的差异无显著性(P>005),而血浆中TNF水平,阿茨海默氏病组明显高于对照组,两组之间比较差异有统计学意义(P<0.05)。结论血清TNF水平显著升高,提示炎性反应在Alzheimer病程中有重要作用,肿瘤坏死因子A-308基因多态性与AD无明显相关。

Objective To investigate the relationship between Alzheimer＇s disease and the plasma level of tumor necrosis factor （TNF） and TNF-α-308G/A gene polymorphism. Method Plasma level of TNF was measured by RIA,and the polymorphism of TNF-α-308G/A gene was screened by PCR-RELP. Result The frequencies of TNF-α-308G/A genotypes in AD group were 0.8636, 0.1212, and 0.0152 for the TNF-α1/1,TNF-α1/2,and TNF-α2/2, respectively. The allele frequencies of TNF-α and TNF-α2 were 0.9242 and 0.0758, respectively. The TNF-α1/1 ,TNF-α1/2, and TNF-α2/2 genotype frequencies of the control group were 0.8881 ,0.1049,and 0.070, respectively. The allele frequencies of TNF-α1 and TNF-α2 were 0.9021, and 0.0979, respectively. There were no significant differences in the genotype frequencies of TNF-α-308G/A gene between the patients and control group （P〉 0. 05）. The plasma level of TNF of Alzheimer＇s disease group was significantly higher than that of control group （P〈 0.05）. Conclusion Plasma TNF level is increased in Alzheimer＇s disease patients and may play an important role in the process of AD. However, no association was found between TNF-α-308G/A gene polymorphism and Alzheimer＇s disease.