蜕皮甾酮对伤口促愈作用的初步研究

Ecdysterone promotes wound healing in rabbits

目的研究蜕皮甾酮对实验性兔创伤模型的促愈作用。方法将21只新西兰兔随机分为3组,每组7只。每只兔制备成含有4个全皮层缺损的创伤模型后,每个创伤缺损分别予以正常对照、地塞米松、蜕皮甾酮、云南白药处理,分别于造模后3、6、9d处死3组兔,取创伤缺损局部组织,常规制片和染色,组织形态学对比观察药物对创伤缺损的作用。结果术后3d,各处理因素创面愈合率比较差异无显著性(P>0.05)。术后6、9d,蜕皮甾酮组、云南白药组分别与地塞米松组比较,差异有显著性(P<0.01);地塞米松组与正常对照组比较,差异无显著性(P>0.05);蜕皮甾酮组与云南白药组比较,差异无显著性(P>0.05)。病理学观察显示,创伤早期各处理因素创面均以渗出和充血为主;术后6、9d可观察到蜕皮甾酮组与云南白药组较其他两组明显促进肉芽组织形成,促上皮细胞增生;蜕皮甾酮组与云南白药组比较,促愈效果相似。结论蜕皮甾酮对实验性兔创伤缺损模型有明显促愈作用,可为临床促愈用药的筛选提供新思路。

Objective To study the efficacy of ecdysterone in promoting wound healing in rabbits. Methods Twenty-one New Zealand rabbits were randomized equally into 3 groups and in each rabbit, 4 separate full-thickness skin wounds were induced and treated with dexamethasone, ecdysterone and Yunnan white powder, or untreated, respectively. On day 3, 6, and 9, respectively, one group of rabbits were sacrificed and the local wound tissue was obtained for pathological examination. Results Three days after the operation, the 4 wounds in each rabbit did not show significant difference in the healing rate （P〈0.05）. On day 6, as was similar to day 9, ecdysterone and Yunnan white powder showed better efficacy in promoting wound healing than dexamethasone （P〈0.01）, the latter having similar effect with the control management （P〉0.05）, and that of ecdysterone was similar to Yunnan white powder （P〉0.05）. Pathologically, early stage of wound healing was characterized by exudation and hyperemia, and on days 6 and 9, ecdysterone and Yunnan white powder exhibited similar strong effet for shaping the granulation tissue and stimulating epithelial cell proliferation. Conclusion Ecdysterone can obviously promote wound healing in rabbits, which may offer a clinical alternative for promoting wound healing.