不同相对分子质量mPEG-SPA修饰血小板CD42a效果差异分析

Effect of platelet CD42a modification by mPEG-SPA with different molecular masses

目的观察mPEG-SPA对血小板抗原的修饰效果,并比较不同相对分子质量的mPEG-SPA修饰效果有无差别。方法分别用相对分子质量为5000、20000的mPEG-SPA对血小板CD42a进行化学修饰;流式法检测修饰前后CD42a荧光强度变化;模拟CD42a三维空间结构,分析赖氨酸区域在CD42a的分布情况。结果经5000和20000的mPEG-SPA修饰后,CD42a荧光强度与对照组相比明显降低,降低比例分别为85.54%和88.65%。CD42a分子表面表达多个赖氨酸区域。结论5000和20000的mPEG-SPA对血小板CD42a均具有良好的化学修饰作用,20000的mPEG-SPA修饰效果优于5000mPEG-SPA的修饰效果。

Objective To observe the effect platelet antigen modification by mPEG-SPA with different molecular masses. Methods Platelet CD42a was modified by 5 kD and 20 kD mPEG-SPA, respectively, and the fluorescence intensity of CD42a was detect by flow cytometry and the three-dimensional structure of CD42a simulated to analyze the distribution of lysine in CD42a molecule. Results After platelet CD42a modification by 5 kD and 20 kD mPEG-SPA, the fluorescence intensity of CD42a decreased sharply by 85.54% and 88.65%, respectively, and multiple lysine regions were identified on the surface of CD42a molecule. Conclusion Both 5 kD and 20 kD mPEG-SPA allow useful modification of platelet CD42a, but 20 kD mPEG-SPA is more advantageous than 5 kD mPEG-SPA.