趋化因子MCP-1在EAN中的作用及雷公藤多甙的影响

Roles of MCP-1 in experimental autoimmune neuritis and response to TWP treatment

目的探讨单核细胞趋化蛋白-1(MCP-1)在实验性变态反应性神经炎(experimental autoimmune neuritis,EAN)中的作用及雷公藤多甙(TWP)对其影响。方法用兔坐骨神经匀浆免疫Wistar大鼠建立EAN模型,TWP灌胃治疗,观察大鼠发病情况和组织病理改变,用免疫组化技术检测MCP-1在坐骨神经中的表达。结果EAN组大鼠在第15天发病达到高峰,且病理改变可见炎性细胞浸润及脱髓鞘,其MCP-1表达高峰在疾病早期(9d),随后逐渐下降,与对照组相比有显著差异性(P<0.001)。EAN+TWP组大鼠发病程度较EAN+NS组轻,MCP-1表达的整体趋势较EAN+NS组降低。结论MCP-1可能对EAN发病起始动作用。TWP可能通过抑制趋化因子MCP-1的表达来减轻EAN。

Object To explore the roles of MCP-1 in experimental autoimmune neuritis （FAN） and responses to TWP treatment. Methods FAN were induced in Wistar rats by immunization with rabbit aeitaic nerves homogenate and complete Freund＇ s adjuvant （CFA）. The immunized rats were administrated intragastrically with TWP daily fill death. The clinical signs of rats and pathological changes in the sciatic nerves of rats were observed. The expression of MCP-1 in the sciatic nerves of rats was detected by immunohistochemistry technology. Results Rats developed EAN with maximum of clinical signs on day 15 after immunization, which was characterized by inflammatory cell infiltration and demyelination in the sciatic nerves. The maximum expression of MCP-1 positive cells in the sciatic nerves was detected on day 9. The expression of MCP-1 in EAN group was obvious higher than that in NS group （ P 〈 0.001 ）. The expression of MCP-1 in the sciatic nerves in EAN ＋ TWP group was lower than that of EAN ＋NS group （P 〈 0.01）. TWP relieved the clinical signs of EAN. Conclusion Chemokine MCP-1 might play an initiatory role in the course of EAN. TWP might relieve the clinical signs of EAN through suppressing the expression of MCP-1.