摘要
衣壳蛋白靶向灭活(CTVI)是一种新型抗病毒策略,它是通过将病毒衣壳蛋白与核酸酶(金黄色葡萄球菌核酸酶、核糖核酸酶Barnase、大肠杆菌RNase HI等)的融合蛋白装配到病毒粒子中,使核酸酶接触并降解病毒核酸,从而达到抑制病毒复制的目的。该策略已经在人免疫缺陷病毒、鼠白血病病毒、乙肝病毒、登革病毒等抗病毒研究中取得良好效果,展示了广阔的应用前景。
Capsid-targeted viral inactivation （CTVI） is a new antiviral strategy, in which nucleases fused to viral coat proteins are expressed in infected cells and become incorporated during virion assembly, and degrade viral nucleic acids. The nucleases commonly used in CTVI are SN nuclease from Staphylococcus, Barnase from Bacillus amyloliquefaciens and RNase HI from E. coli. CTVI has successful applications in HIV-1 hepatitis B virus, murine leukaemia virus, Dengue virus, etc. The principle, advantages, applications and prospect of CTVI were mainly described.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第3期88-92,共5页
China Biotechnology
基金
国家973计划资助项目(2005CB523202)
