犬细小病毒2b亚型VP2基因的克隆及其B细胞抗原表位分析

Cloning of VP2 gene of canine parvovirus 2b subtype and analysis of antigen epitopes on B cell of deduced amino acid sequence

对犬细小病毒2b亚型衣壳蛋白VP2基因进行了克隆、测序，并用分子生物学软件DNAStar对VP2基因的推导氨基酸序列进行了表位分析。结果显示，获得了犬细小病毒2b亚型的VP2基因，序列全长1755bp，编码584个氨基酸；B细胞表位主要位于VP2蛋白第1～38、73～83、86～104、152～195、235～243、294～326、347～400、404～416、469～483、503～521和571～585区段。表明，犬细小病毒2b亚型VP2蛋白上分布有多个B细胞抗原表位，这为犬细小病毒基因工程疫苗的研制奠定了基础。

To predict antigen epitopes on B cell of VP2 of canine parvovirus 2b subtype（CPV-2b）,the VP2 gene of CPV-2b was cloned and sequenced. The antigenicity of VP2 was analysed by DNAStar software. The results showed that the complete sequence of the VP2 gene was 1 755 bp in length encoding for 884 amino acids, and many distinct antigen epitopes were situated in the VP2 regions 1--38,73--83,86-- 104,152--195,235--243,294--326,347--400,404--416,469--483,503--521 and 571--585. It is concluded that the capsid VP2 containes many B cell antigen epitopes, which lays down the foundation for development of engineering vaccines against CPV.