摘要
目的观察1,25-二羟基维生素D3[1,25-(OH)2D3]对体外人牙周膜细胞(HPDLC)破骨细胞分化因子(ODF)mRNA及破骨细胞发生抑制因子(OCIF)mRNA表达的影响,探讨正畸牙周组织改建的分子机制。方法以不同浓度的1,25-(OH)2D3(0、1×10-10、1×10-8、1×10-6mol/L)作用于体外培养的人牙周膜细胞,利用原位杂交染色及RT-PCR检测技术,检测不同浓度的1,25-(OH)2D3作用于体外培养人牙周膜细胞ODF、OCIFmRNA表达的强度变化。结果随1,25-(OH)2D3浓度的升高,人牙周膜细胞ODFmRNA的表达升高,而OCIFmRNA的表达降低。结论1,25-(OH)2D3在体外可影响人牙周膜细胞ODFmRNA和OCIFmRNA的表达,从而通过调节ODF和OCIF相对含量的变化,影响牙周组织改建过程。
Objective To clarify the molecular mechanism of periodontal tissue remodeling during orthodontic tooth movement by investigating the effects of 1,25-（OH）2D3 on the expression of ODF and OCIF in hmnan periodontal ligament cells （HPDLCs）. Methods HPDLCs were subjected to different doses of 1,25-（OH）eD3（0, 1 × 10^-10,1 × 10^-8, 1 ×10^-6 mol/L） for 24 hours in this experiment, mRNA expression of ODF and OCIF were detenninod by semi quantitative RT-PCR approach. Results The expression of ODF mRNA and increased significantly in a dose-dependent manner,and the expression of OCIF mRNA didn＇t decrease not significantly. Conclusion 1,25-（ OH）eD3 could adjust the proportion of expression of ODF to expression of OCW. Thus, the hone metabolism was adjusted.
出处
《口腔医学》
CAS
2007年第2期57-60,共4页
Stomatology
基金
国家自然科学基金资助项目(30300393)
