半导体激光诱导大鼠脉络膜新生血管模型的实验研究

A diode laser-induced choroidal neovascularization model in Brown Norway rats: an experimental study

目的:评价532nm半导体激光诱导棕色挪威(brown norway,BN)大鼠脉络膜新生血管(choroidal neovasularization,CNV)模型的可行性。方法:使用半导体激光(功率150 mW,光斑直径75μm,曝光时间0.1 s)对10只BN大鼠建立CNV模型。分别于光凝后7、14、21和28 d随机选取5只行眼底荧光素血管造影术(fundus fluorescein angiogrphy,FFA)和光学相干断层成像术(optical coherence tomography,OCT)检查,比较FFA早期(<2 min)和晚期(>10 min)荧光渗漏斑数/平均渗漏面积(mm2)的变化,并行统计学分析。结果:光凝后FFA检查经组间两两比较,142、1和28 d的早期荧光渗漏斑数/渗漏面积差异无统计学意义(P>0.05),14、21和28 d与7 d相比差异有统计学意义(P<0.05);7、14、21和28 d早期和晚期荧光渗漏斑数差异无统计学意义(P>0.05)。激光斑视网膜的平均厚度与平均荧光渗漏面积有相关性(r=0.73,P<0.05)。结论:半导体激光诱导BN大鼠的CNV模型是可行的。FFA联合OCT可有效检测CNV的形成和变化。

Objective： To evaluate the feasibility of the diode laser in inducing choroidal neovascularization （CNV） in Brown Norway （BN） rats. Methods： Ten diode laser spots （75 μm, 0.1 s and 150 mW） were applied to one fundus around the optic disc of 10 BN rats. The same spot of CNV formation was determined by fundus fluorescein angiography （FFA） and optical coherence tomography （OCT） on the 7, 14, 21 and 28 d after laser photocoagulation. The changes of the number/area ratio of fluorescein leakage（mm^2） at the early-phase （ 〈 2 minutes） and the late-phase （ 〉 10 minutes） of each day were measured. Results： The number/mean area ratio of the fluorescein leakage had not a statistically significant difference among the early FFA phase on the 14, 21 and 28 d （ P 〉 0.05）, but a significant dif- ference between the 14, 21 and 28d and the 7 d （ P 〈0.05）. The number of the fluorescein leakage at the early-phase of 7, 14, 21 and 28 d was not significantly different from that at the late-phase （ P 〉 0.05）. The correlation of the mean area of the fluorescein leakage and the mean retinal thickness was obvious（ r =0.73, P 〈0.05）. Conclusion： The laser-induced rat is suitable for studying CNV in vivo. FFA combined with OCT is better for detecting the formation and the changes of CNV.