移植肾急性排斥前后外周血淋巴细胞CD15S抗原表达的变化

Expression of CD15S antigen on peripheral lymphocytes before and after acute rejection following renal transplantation

目的:研究移植肾急性排斥前后及肾功能稳定患者外周血淋巴细胞CD15S抗原表达及意义。方法:70人(95例次)分为术后稳定组(组1,n=45)、急性排斥反应组(组2,n=25)及抗排斥治疗逆转组(组3,n=25)。应用流式细胞技术检测三组外周血淋巴细胞CD15S抗原的表达,观测各组的CD15S阳性细胞率。对各组间观测值之间的差异用独立样本t检验进行分析。结果:组2表达显著高于组1和组3(P<0.01),组1和组3间差异无统计学意义(P>0.05)。结论:抗排斥治疗逆转后,CD15S表达降低至术后稳定组患者水平,监测该抗原可诊断急性排斥反应,并可提示抗排斥治疗的效果。

Objective： To investigate the difference of CD15S antigen expression on peripheral lymphocytes and its clinical significance in patients before and after acute rejection and of those with a stable graft function after renal transplantation. Methods： Flow cytometry was used to detect the expression of CD15S antigen on peripheral lymphocytes in 70 patients（ 95 specimens）. These subjects were divided into 3 groups： patients with a stable graft function （group 1）, patients with acute rejection （group 2） and patients recovered after an anti-rejection therapy （group 3）. There were respectively 45, 25 and 25 subjects in each group. The percent of CD15S positive lymphocytes of each subject was detected. CD15S expression of every group was compared with a Student t test. Result： The data of group 2 were significantly higher than those of the other 2 groups, and there were no significant differences between group 1 and 3. Conclusions： CD15S expression of group 3 is equal to that of group 1. Monitoring of CD15S antigen expression on peripheral lymphocytes after renal transplantation may contribute to the diagnosis of acute rejection and may predict the effect of an anti-rejection therapy.