兔VX_2肝癌模型制作及其影像学分析

Different VX_2 liver tumor in rabbits and its image features

目的:评价不同方法制作兔VX2肝癌模型种植率,分析兔VX2肝癌影像学表现。方法:将新西兰大白兔60只分为开腹包埋、开腹穿刺及直接经皮穿刺种植三组(每组20只),每组又分为10只瘤块接种,10只悬液接种。接种2周后,以CT、MRI检测肿瘤种植及生长状况,然后行肝动脉造影检查。结果:开腹包埋、开腹穿刺接种种植率差异无统计学意义(P>0.05),开腹与经皮穿刺比较,差异有统计学意义(P<0.05)。瘤块接种比悬液接种种植率高(P<0.05)。CT平扫,VX2肿瘤呈低密度,动脉期环性强化,门脉期呈低密度。MRI平扫呈长T1,稍长T2信号。肝动脉造影示肿瘤富血供,由肝动脉供血。接种3周后,肿瘤易出血坏死。结论:开腹包埋与开腹穿刺接种制作VX2兔肝癌模型简单,实验周期短,接种率高。开腹瘤块法复制兔VX2肝癌模型,其影像学表现类似人类原发性肝细胞肝癌,可进行进一步的临床实验研究。

Objective： To evaluate the success rate of VX2 tumor in rabbits and study the image manifestations by different methods. Methods： VX2 tumor was implanted in 60 New Zealand rabbits by three methods： laparotomic burying, laparotomic puncture and percutaneous puncture, 20 rabbits per group. Ten were subjected to carcinoma masses and ten carcinoma fluids in each group. CT, MRI and liver angiography were performed two weeks after inoculation. Resuits： Implantation rate was significantly higher in the two laparotomic routes compared with the percutaneous route. The VX2 liver tumors were demonstrated as hypodensity on plain CT scanning and enhanced at an early artery phase and hypodensity at a portal phase image. On MR scannings, the VX2 liver tumors were seen as hypointensity on T1WI and slight hyperintensity on T2WI. Hepatic angiography showed the tumors were hypervascular and the hepatic artery was the main feeding vessel. Necrosis and bleeding were often found in the area of VX2 liver tumors two weeks after implantation. Conclusion： Construction of VX2 liver tumor by a laparotomic route is simple and short circled. The image manifestations of the VX2 liver tumor in rabbits are similar to those of hepatic primary carcinoma in human.