HIV整合酶抑制剂体外筛选方法研究进展

The Methods for Screening HIV Integrase Inhibitors in vitro

为了评价HIV整合酶抑制剂体外筛选方法的特点和整合酶抑制剂研究进展,以常用的3′加工反应法、链转移反应法、改良ELISA法及基于结构的筛选为基础归类整合酶抑制剂。整合酶抑制剂按方法分为五类,并可推测其作用机制,通过比较可知以上方法各有利弊,需配合运用。整合酶抑制剂有研究价值。

The advantages and characteristic of the methods for screening HIV integrase inhibitors in vitro and the development of integrase inhibitors were evaluated. Through particularizing method such as 3＇ process reaction, chain transfer reaction, improved ELISA, and screening inhibitors based on structure, HIV integrase inhibitors were classified. HIV integrase inhibitors can be divided into five species and used to speculate their mechanisms. All these methods should be cooperatively applied, and inh.ibitors have a research value.