贵州野生多汁乳菇对小鼠免疫功能影响

Efects of wild lactarius volemus from Guizhou province on immunological function in mice

目的观察贵州野生多汁乳菇对正常及环磷酰胺(CP)损伤免疫系统小鼠的免疫调节作用。方法实验设多汁乳菇组9.0 mg/(kg.bw)、多汁乳菇组9.0 mg/(kg.bw)+CP、CP组、正常对照组。灌胃给小鼠多汁乳菇提取液35 d,CP腹腔注射造成小鼠免疫损伤。观察小鼠体重的生长情况、小鼠脏器系数、血清溶血素、小鼠足趾迟发型超敏反应(DTH)、碳粒廓清指数α等指标。结果小鼠胸腺系数、血清溶血素(HC50)、小鼠足趾DTH、碳粒廓清指数α明显高于CP组(P<0.01)。其中小鼠足趾DTH,碳粒廓清指数α恢复至正常对照组水平。结论贵州野生多汁乳菇对CP所致的免疫损伤小鼠的细胞免疫、体液免疫、非特异性免疫功能有明显的改善作用。

Objective To observe the immunological effects of wild lactarius volemus on normal and the immune deficit mice induced by cyclo- phos- phamide（CP）. Methods Mice were randomly divided into 4 groups.- 2 test groups administered with lactarius volemus [9.0 g/（kg·bw）] and lactarius volemus [9.0 g/（kg·bw）] ＋ CP, negative control group, and positive control group（CP） . 35 days later, the five indicators were determined, respectively. Results Thymus index of mice, HC50 in serum, delayed type hypersensltivity（DTH）, and the cabon clear index（α） of cabon clear test in lactarius volemus [9.0 g/（ kg. bw）3 ＋ CP groups were significantly more than that in positive control group（CP） （ P 〈 0.01 ）. The delayed type hypersensitivity（DTH） and the cabon clear index（α）, restored the normal test group level. Conclusion The wild lactarius volemus can protect the mice from the damage of immunological function including humoral mediated immunity, cellar mediated immunity and macrophage function by cyclo- phos- phamlde.