铝螯合剂对染铝大鼠肝酶谱及必需元素影响

Effect of aluminum chelating agent on liver zymogram and on essential elements of rats induced by aluminum

目的探讨铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(DHPO)对铝染毒大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)及肝脏中铝、锌、铜、铁、钙、镁等元素的影响。方法AlCl3染毒Wistar大鼠3周后分别给予不同剂量的DHPO 1周,测定血清ALT、AST、ALP活力及肝脏中铝、锌、铜、铁、钙、镁等元素含量。结果各组大鼠的ALT活力比较差异均无统计学意义(P>0.05);低、中、高剂量组的AST活力显著低于模型组(P<0.01);低剂量组ALP活力与模型组比较差异有统计学意义(P<0.01);Al含量测定结果显示:低、中、高剂量组均显著低于模型组(P<0.01),且含量呈下降趋势,中、高剂量组的大鼠肝脏中锌、铜、铁、钙、镁的含量与阴性对照组比较差异均无统计学意义(P>0.05)。结论DHPO在促进排铝的同时,对肝脏中锌、铜、铁、钙、镁含量无影响,且在一定剂量范围内对AST、ALP活力具有恢复作用。

Objective To inquire into the effects of 1,2 - dimethyl - 3 - hydroxypyrid- 4 - pyridine（DHPO） on alanine aminotransferase（ALT）, glutamic oxalacetic transaminase（AST）, and alkaline phosphatase（ALP） in serum and elements aluminum（Al）, zinc（Zn）, copper（Cu）, iron（Fe）, calcium（Ca） and magnesium（Mg） in liver of rats induced by aluminum. Methods Wistar rats were administered with aluminum chloride for 3 weeks. Then they were treated with different doses of DH- PO. The activity of ALT, AST and ALP in serum was detected, and the concentrations of Al, Zn, Cu, Fe, Ca and Mg in liver tissue were measured. Results The result of ALT activity demonstrated that there was no statistical significance among all the groups（ P 〉 0.05 ）. The AST activity of low, moderate and high DHPO dose groups was lower than the positive control（ P 〈0.01）. The result of ALP activity indicated that the low DHPO dose group was significantly different from the positive control（ P〈 0.01 ）. The aluminum concentration of the three different dose groups was significantly lower than the positive control（ P 〈 0, 01 ）. Compared with the negative control, the Zn, Cu, Fe, Ca, Mg concentrations of the moderate and high DH- PO dose groups were not significantly different（ P 〉 0.05）. Conclusions DHPO could effectively enhance the elimination of Al in liver, but dose not affect the excretion of Zn, Cu, Fe, Ca and Mg. DHPO can recover the activity of AST and ALP in serum being damaged by aluminum in some degree.