期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

亚砷酸钠对血红素加氧酶-1诱导作用 被引量：1

Sodium arsenite-induced expression of heme oxygenase-1 in bovine aortic endothelial cells
下载PDF
导出
摘要 目的观察不同暴露剂量和时间下,亚砷酸钠(NaAsO2)对体外培养的牛主动脉血管内皮细胞(BAEC)中血红素加氧酶-1(HO-1)的诱导作用。方法分别在0-15μmol/L NaAsO2暴露0-24 h收集培养的BAEC细胞蛋白提取液中,用蛋白印迹(western blot)法检测HO-1蛋白的诱导产生量。结果0-15μmol/L NaAsO2暴露能够显著诱导BAEC细胞HO-1蛋白产生增加,并且分别具有剂量-反应和时间-反应相关关系;2.5μmol/L NaAsO2暴露即可显著诱导产生HO-1蛋白。结论HO-1蛋白的诱导表明砷介导的一种细胞应激反应;HO-1蛋白可以作为无机砷暴露的敏感性生物标志物之一,有利于深入研究无机砷暴露的生物剂量反应。 Objective To observe sodium arsenite （NaAsO2） -induced expression of heme oxygenase- 1 （HO- 1）in bovine aortic endothelial cells （BAEC）, Methods Cultured BAEC was exposed to NaASO2 （0- 15μmol/L） for 0- 24 h, respectively. Western blot was used to detect the induction of HO- 1 protein. Results 0 15 μmol/L NaAsO2 exposure induced the production of HO - 1 protein significantly, with dose - dependent and time - dependent relationship, Low dose of NaAsO2（2.5 μmol/L） could induce markedly the expression of HO - 1 protein, Conclusion HO - 1 induction could be a cellular stress response, and has potential for being used as a sensitive biomarker for inorganic arsenic exposure, which may be useful for further research of the biological response of arsenic.
作者 李冰 宫慧芝 孙鲜策 孙贵范
机构地区 中国医科大学公共卫生学院 大连医科大学
出处 《中国公共卫生》 CAS CSCD 北大核心 2007年第3期340-341,共2页 Chinese Journal of Public Health
基金 国家自然科学基金(30600510)
关键词 亚砷酸钠 血红素加氧酶-1(HO-1) 血管内皮细胞 sodium arsenite heme oxygenase - 1 （ HO - 1 ） vascular endothelial cell
分类号 R995 [医药卫生—毒理学]
  • 相关文献

参考文献9

  • 1Sun GF.Arsenic contamination and arsenicosis in China[J].Toxicol Appl Pharmacol,2004,198(3):268-271.
  • 2Elbirt KK,Bonkovsky HL.Heme oxygenase:recent advances in understanding its regulation and role[J].Proc Assoc Am Physicians,1999,111(5):438-447.
  • 3Kumagai Y,Pi J.Molecular basis for arsenic-induced alternation in nitric oxide production and oxidative stress:implication of endothelial dysfunction[J].Toxicol Appl Pharmacol,2004,198 (3):450 -457.
  • 4Michel T,Li GK,Busconi L.Phosphorylation and subcellular translocation of endothelial nitric oxide synthase[J].Proc Natl Acad Sci USA,1993,90(13):6252-6256.
  • 5李冰,王毅,孙贵范.甲基亚砷酸对内皮型一氧化氮合酶磷酸化影响[J].中国公共卫生,2007,23(1):98-99. 被引量：4
  • 6Gong P,Stewart D,Hu B,et al.Multiple basic-leucine zipper proteins regulate induction of the mouse heme oxygenase-1 gene by arsenite[J].Arch Biochem Biophys,2002,405 (2):265-274.
  • 7Liu J,Kadiiska MB,Liu Y,et al.Stress-related gene expression in mice treated with inorganic arsenicals[J].Toxicol Sci,2001,61(2):314-320.
  • 8Hirano S,Ciu X,Li S,et al.Difference in uptake and toxicity of trivalent and pentavalent inorganic arsenic in rat heart microvessel endothelial cells[J].Arch Toxicol,2003,77(6):305-312.
  • 9Lee PC,Ho IC,Lee TC.Oxidative stress mediates sodium arsenite-induced expression of heme oxygenase-1,monocyte chemoattractant protein-1 and interleukin-6 in vascular smooth muscle cells[J].Toxicol Sci,2005,85 (1):541-550.

二级参考文献5

  • 1Vasken Aposhian H, Zakharyan RA, Avram MD, et al. A review of the enzymology of arsenic metabolism and a new potential role of hydrogen peroxide in the detoxication of the trivalent arsenic species [J]. Toxicol Appl Pharmacol, 2004, 198 : 327 - 335.
  • 2Souza K, Meddock DA, Zhang Q, et al. Arsenite activation of P13K/ AKT cell survival pathway is mediated by p38 in cultured human keratinoeytes[J]. Mol Med, 2001, 7:767 - 772.
  • 3Hossain K, Akhand AA, Kawamoto Y, et al. Caspase activation isaccelerated by the inhibition of arsenite-induced, membrane raftsdependent Akt activation[ J ]. Free Radic Biol Med, 2003, 34: 598 -606.
  • 4Kumagai Y, Pi J. Molecular basis for arsenic-induced alteration in nitric oxide production and oxidative stress:impllcation of endothelial dysfunction[ J]. Toxieol Appl Pharmeol, 2004,198:450 - 457.
  • 5McCabe TJ, Fulton D, Roman LJ, et al. Enhanced dectron flux and reduced ealrnodulin dissociation may explain"calcium-independent"eNOS activation by phosphorylation [J ]. J Biol Chem, 2000, 275:6123 - 6128.

共引文献3

同被引文献13

  • 1胡娟.低氧刺激与机体适应性应激蛋白研究进展[J].中国公共卫生,2004,20(5):621-623. 被引量：3
  • 2田英平,苏建玲,高恒波,佟飞,陈慧,石汉文,霍书花.113例百草枯中毒救治体会[J].中国急救医学,2006,26(7):542-543. 被引量：149
  • 3廖润玲,杨斌.一氧化氮及诱导型一氧化氮合酶的研究进展[J].时珍国医国药,2007,18(4):980-981. 被引量：38
  • 4Soslow RA,Dannenberg AJ,Rusla D,et al. Cox-2 is expressed in human pulmonary, colonic, and mammary tumors [ J ]. Cancer, 2000,89:2637 - 2645.
  • 5Choe W, Kin S, Hwang TS, et al. Expression of inducible nitric oxide synthase in thyroidneoplasns immunoistochemieal and molecular analysis[ J ]. Pathol Int,2003,53 (7) :434.
  • 6Stuehr D, Pou S, Rosen GM. Oxygen reduction by nitric-oxide synthase[J]. Biol Chem,2001,276(18) :14533 - 14536.
  • 7BonneU MR, Visner GA, Zander DS, et al. Heme oxygenase-1 expression correlates with severity of acute cellular rejection in lung transplantation [ J ]. J Am Coil Sur, 2004,198 ( 6 ) : 945 - 952.
  • 8Tomita M,Okuyama T, Katsuyama H, et al. Paraquat-induced gene expression in rat kidney [ J I. Arch Toxicol,2006,80 ( 10 ) : 687 - 693.
  • 9Demple B. Protection from the dark side of NO: signaling and cellular defenses against nitric oxide toxicity [ J ]. IUBMB Life, 2004:59 - 64.
  • 10Tanaka S, Akaike T, Fang J, et al. Antiapoptotic effect of haem oxygenase-1 induced by nitric oxide in experimental solid turnout [ J]. Br J Cancer,2003,88:902 - 909.

引证文献1

中国公共卫生
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部