利用重组近交系群体检测花生青枯病抗性SSR标记

Identification of SSR markers linked to bacterial wilt resistance of peanut with RILs

用抗青枯病花生品种远杂9102与感病品种Chico杂交,从F2起用单粒传法构建了花生重组近交系群体(RIL)F6和F7。采用354对SSR引物对重组近交系F6群体的基因组DNA鉴定,获得多态性标记45个。结合重组近交系群体F6和F7青枯病抗性鉴定结果,应用相关软件统计分析,构建了栽培种花生部分遗传连锁图。图谱总长度为603.9cM,含29个标记(28个SSR标记和1个表型标记)的8个连锁群,还有17个独立的SSR标记;获得了与青枯病抗性相关的SSR标记2个(7G02和PM137),位于该图谱的第1连锁群上,与青枯病抗性基因间的遗传距离为10.9cM和13.8cM,并且位于抗性基因的两侧,两标记间的距离为23.7cM。

Bacterial wilt （BW） caused by Ralstonia solanacearum is an important constraint to peanut （Arachis hypogaea L. ） production in several Asian and African countries and host plant resistance is crucial for controlling this disease. The available released BW - resistant peanut cultivars in China are of relatively low yielding with poor seed quality and poor resistance to other constraints. Molecular markers linked to bacterial wilt resistance genes would facilitate efficient pyramiding of genes related to disease resistance and yield as well as seed quality. In the present study, resistant line Yuanza 9102 was crossed with susceptible line Chico. Recombinant inbred lines （RILs） through single seeded descent method were developed. The F6 RILs was detected by SSR techque through 354 primers. Fourty -five SSR markers which could test the polymorphism among the F6 lines were identified. Based on the results through bacterial wilt resistance evaluation of F6 and F7 RILs and DNA marker, a map including 8 linkage groups covering a map distance of 603.9 cM containing 29 markers （28 SSR markers and 1 phenotypic marker） and 17 unique marker was eveloped. Two SSR markers, 7G02 and PM137 were identified as related to bacterial wilt resistance and reaped on linkage group 1 and in the different side of the resistant gene. The distance between the two markers and resistant gene were 10.9 cM and 13.8 cM, respectively.