汞和镍对人血淋巴细胞转化及DNA合成的效应

Effects of mercuric and nickel on blast transformation and DNA synthesis of human blood lymphocytes

通过测定３Ｈ－ＴｄＲ参入细胞ＤＮＡ的方法研究了氯化汞和氯化镍对人血淋巴细胞转化和ＤＮＡ合成的效应。结果表明，氯化汞和氯化镍对人外周血淋巴细胞的转化和ＤＮＡ合成的效应是双相性的：在极低浓度下汞、镍化合物均可促进淋巴细胞转化和ＤＮＡ合成，而在高浓度下则抑制淋巴细胞转化和ＤＮＡ合成。汞、镍化合物对淋巴细胞的这种刺激效应比细胞有丝分裂素—植物血凝素（ＰＨＡ）的刺激效应要弱。

Effects of mercuric chloride and nickel chloride on blast transformation and DNA synthesis of unsensitized human blood lymphocytes were investigated with determination of 3 H-thymidine ( 3H TdR)incorporation into cellular DNA. The results have shown that effects of mercuric chloride nickel chloride on blast transformation and DNA synthesis of human blood lymphocytes are biphasic:the chemicals at very low concentrations enhance the blast transformation and DNA synthesis, whereas higher concentrations inhibite these processes. The stimulating effects of mercuric and nickel on human lymphocytes are weaker than the lectinic mitogen-PHA The stimulating effect of mercuric on the blast transformation and DNA synthesis is stronger than that of nickel.