微生态制剂EM对蜜蜂微孢子体外发芽抑制实验

Inhibition of Microbial Ecological Agents on Germination of Nosema apis in Vitro

防治蜜蜂孢子病的关键是抑制蜜蜂微孢子发芽。分四部分对此进行了探索:第一部分探索了分离纯化蜜蜂微孢子的方法,试验证明Percoll法分离的孢子均匀一致,无杂质和杂菌,适合后期试验要求;第二部分探索了蜜蜂微孢子人工发芽的最佳方法,试验证明KOH法孢子发芽率高,污染率低,为最佳的发芽方法;第三部分探索了蜜蜂微孢子人工发芽的最佳培养基,试验证明pH7.8的PBS发芽率最高,为最佳发芽培养基;第四部分探索了EM对蜜蜂微孢子体外发芽抑制作用,试验证明EM对蜜蜂微孢子体外发芽有较强的抑制的作用,浓度越高,抑制效果越好。

The key to prevent Nosema disease is to inhibit Nosema apis Zander spore germinating, so the authors studied it from four parts. In the first part, the method for purifying N. apis spore was approached. The result showed that the purified spore by Pereoll method was symmetrical, no impurity and microbial. In the second part,the best way for artificial germination of N. apis spore was approached. The result showed that the germination ratio was high and the contamination was reduced by the method of KOH. In the third part, the best culture medium for artificial induction of N. apis spore was selected. The result showed that the germinating ratio was the highest with PBS of pH7.8. In the fourth part, the inhibition of EM on germination of Nosema apis in vitro was studied. The inhibition of EM on germination of Nosema apis in vitro was very strong, and the higher the concentration of EM the better effect of the inhibition.