斑马鱼体表类穴位与类经络结构的初步观察

Preliminary observation of acupoint-like and meridian-like structures on the body surface of the zebrafish

目的:探讨斑马鱼是否存在类经络与类穴位结构。方法:将成年斑马鱼活体经10μmol/LDiA水溶液染色,麻醉后在荧光显微镜下观察。结果:DiA荧光标记物呈亮圆点状,若干标记物聚集在一起形成簇状,有规律地分布于身体各个部位。在躯体部,标记物从背部到腹部沿躯干纵轴形成4条线,背侧线、背外侧线、外侧线和腹侧线。在高倍镜下观察,又可依据其神经联系的不同将外侧线分为外侧上线和外侧下线,将腹侧线分为腹外侧线和腹侧线。结论:斑马鱼体表感觉器官分布有一定的规律性,若干感觉器官聚集在一起形成簇,若干簇又有规律地沿躯体长轴排成线,类似于传统经络与穴位。

Objective To probe whether there are acupoint-like and meridian-like structures on the body surface of the zebrafish. Methods Adult zebrafish were put into a 10 μmol/L DiA water solution and then anaesthetized for observation under a fluorescent microscope. Results The labeled substance appeared in round bright spots and some of them gather together to form a cluster which distributed through the body surface orderly. The labeled clusters along the long axis of the body distributed in four lines, dorsal line, dorsal lateral line, lateral line and ventral line. The lateral line could be divided into two lines, lateral superior line and lateral inferior line; and the ventral line into ventral lateral line and ventral line based on their nerve connection under high power microscope. Conclusion Sensory organs of the zebrafish distributed orderly and formed clusters appearing orderly in lines throughout the body surface, which are much like acupoints and meridians described in traditional Chinese medicine.