Ⅰ-型胰岛素样生长因子基因转移对烫伤大鼠创面愈合作用的实验研究

Experimental study of gene transfer of insulin-like growth factor Ⅰ on wound healing of scald rats

目的探讨Ⅰ-型胰岛素样生长因子（insulin-like growth factor Ⅰ，IGF-Ⅰ）基因转移对烫伤大鼠创面愈合的作用效果。方法利用我们成功构建的重组真核表达质粒pcDNA3．1／IGF-Ⅰ，通过脂质体介导转染烫伤大鼠皮肤组织，用免疫组化方法检测IGF-Ⅰ基因在皮肤及肝脏组织的表达情况，观察烫伤大鼠体重、刨面愈合情况等指标。结果免疫组化结果显示，脂质体介导的IGF—Ⅰ基因转移可在创面注射点周围皮肤成纤维细胞中出现阳性表达，而肝脏组织中未出现阳性表达，实验组大鼠创面注射IGF-Ⅰ重组质粒后，创面愈合与对照组比较差异有统计学意义（P〈0．05），实验组大鼠体重与对照组大鼠比较未出现减轻现象（P〈0．05）。结论脂质体介导IGF-Ⅰ基因转移，可促进创面愈合，并可避免IGF-Ⅰ全身应用所带来的副作用。

Objective To probe into the effect of IGF-Ⅰ gene transfer on wound healing of scald rats. Methods Eukaryotic recombinant plasmid of pcDNA3.1/ IGF-Ⅰ （successfully constructed by our study group earlier） transported by liposeme were injected subdermally in wound region of scald rats. Changes of body weight and tendency of wound healing were observed carefully within 5 weeks postburn. The expression of IGF-Ⅰ gene transfer in serum and liver was observed with immunohistochemical staining. Results The results of animal experiment showed that IGF-Ⅰ gene transported by liposome expressed positively in fibroblast in burn wound, but expressed negatively in liver tissue with immunohistochemical staining, There were no significant differences for IGF-Ⅰ concentrations in serum among treatment group and control group （ P 〉 0.05）. The speed of wound healing for animals injected with IGF-Ⅰ reconstruct was faster than those which didn＇t injected IGF-Ⅰ （ P 〈 0.05） ; The body weight of the animals in the group injected with IGF-Ⅰ hadn＇t a loss in 5 weeks postburn, but those which didn＇t injected IGF-Ⅰ had a loss in body weight during post burn days （ P 〈 0.05 ）. Conclusions Gene transfer of IGF-Ⅰ transported by liposeme can promote wound healing, and it also could avoid some adverse effect brought by application of IGF-Ⅰ systematically .