人类巨细胞病毒编码趋化因子HCMV UL146序列在临床低传代分离株中的多态性研究

Sequence variability of the chemokine encoded by human cytomegalovirus

目的探讨人类巨细胞病毒（human cytomegalovims，HCMV）UL146序列在临床患儿低传代分离株中的多态性及其与临床疾病的关系。方法对23株HCMV临床低传代分离株及2例同年龄组HCMV-DNA定量PCR方法检测阳性健康儿尿液进行HCMV-UL146 PCR扩增及测序分析。结果UL146序列呈现较高的多态性，UL146的序列可以分为3组，所有巨结肠患儿标本均分布在G2组，无症状感染儿均在G2B组，而α化学因子功能域在各序列中保守。结论序列的变异可能会影响UL146吸附中性粒细胞，影响病毒扩散。

Objective To study the sequence variability of UL146 ORF in HCMV clinical isolates and examine the possible associations between gene variability and the outcome of HCMV infection. Methods UL146 gene from strains obtained from suspected congenitally HCMV infected infants were PCR amplified and sequenced. Results High variability was found in UL146 gene among HCMV clinical strains. However the a chemokine motif in UL146 gene was conserved in almost all sequences. According to the phylogenetic analysis, all sequences of UL146 in clinical isolates could be divided into three groups. All strains from congenital megacolon infants existed in G2 only, and all from asymptomatic infants existed in G2B peculiarly. Conclusions Sequence variability among HCMV clinical strains maybe affect the ability of UL146 to attract human neutrophils and influence viral dissemination. No obvious linkage was observed between UL146 polymorphisms and outcome of suspected congenital HCMV infection.