变应性鼻炎大鼠翼腭神经节内NOS和NGF的表达变化

Expression of nitric oxide synthases and nerve growth factor in pterygopalatine ganglion of the allergic rhinitis rats

目的:观察一氧化氮合酶(NOS)和神经生长因子(NGF)在变应性鼻炎(AR)大鼠翼腭神经节内的表达变化。方法:建立AR大鼠模型,用HE染色方法观察鼻黏膜内腺体的变化;用组织化学的方法观察翼腭神经节内NOS表达的变化;用免疫荧光的方法观察翼腭神经节内NGF表达的变化。结果:实验组和正常组大鼠翼腭神经节内均有NOS和NGF表达,但实验组大鼠翼腭神经节内NOS和NGF表达均较正常组显著性增加(P<0.01)。结论:NOS和NGF可能通过神经调节机制参与了AR的致病过程。

Objective：To investigate the expression level of nitric oxide synthases（NOS） and nerve growth factor（NGF） in pterygopalatine ganglion of the allergic rhinitis rats. Methods： The expression level of NOS and NGF was detected in pterygopalatine ganglion of the allergic rhinitis rats using histochemical and immunofluorescence staining methods respectively. Results：The number of labelled NOS-positive neurons and NGF-positive neurons was respectively increased significanfly（P 〈 0.01 ） in pterygopalatine ganglion of the allergic rhinitis rats. Conclusion：NOS and NGF were probably involved in the pathologic mechanisms of the allergic rhinitis by the nerve regulation.