促红细胞生成素对大鼠骨髓间充质干细胞增殖和细胞周期的影响

Effects of erythropoietin on proliferation and cell circle stage of rat bone marrow mesenchymal stem cells in vitro

目的:观察促红细胞生成素(eryrthropoietin,EP)对大鼠骨髓间充质干细胞(mesenchymalstemcells,MSCs)增殖和细胞周期的影响。方法:无血清条件下,不同剂量(0,0.25,0.5,1,2,10U/ml)EP与MSCs共培养24h,MTT方法检测MSC增殖情况;10U/mlEP与MSCs共培养72h后,流式细胞仪检测细胞周期情况。结果:MTT结果表明,EP呈剂量依赖性促进MSCs增殖,除0.25U/ml剂量外,其余各组都与对照组有显著性差异,以10U/mlEP促MSCs增殖作用最明显。流式细胞检测表明,EP组能降低G0/G1期比例,增加S期和G2/M期细胞比例,与对照组相比,差异均有显著性意义(P<0.05)。结论:EP可以促进骨髓间充质干细胞增殖,并且这种影响呈剂量依赖性。

Objective：To determine the effect of erythropoietin （EP） on the proliferation of mesenchymal stem cells （MSCs） in vitro. Methods：Under serum free culture media, MSCs were incubated with different doses of EP for 24 h （0, 0.25, 0.5, 1, 2, 10 U/ml）, and then the proliferation of MSCs was measured by MTT assay. Cell circle stage was measured by flow cytometry assays after incubation of 10 U/ml EP for 72 h. Results：MTT assay showed that EP could enhance MSCs proliferation in a dose-dependent manner. There was significant difference among EP treatment groups and control group,except 0.25 U/ml group. The effects on the proliferation of MSCs was highest in 10 U/ml group. Furthermore,flow cytometry （FCM） assays showed that, compared with control group, EP could significantly decrease G0/G1 ratio, and increase S stage and G2/M stage ratio. Conclusion：EP could enhance MSCs proliferation in dose-dependent manner.