谷胱苷肽转硫酶GSTM1、GSTT1和GSTP1基因多态性与江苏人群直肠癌易感性的关系

Polymorphisms of GSTM1, GSTT1 and GSTP1 and susceptibility of rectal cancer

目的:探讨谷胱苷肽转硫酶M1、T1、P1(GSTM1、GSTT1和GSTP1)基因多态性和吸烟饮酒习惯与直肠癌易感性的关系。方法:以直肠癌患者210例,人群对照439例为研究对象,调查研究对象的生活习惯,以多重PCR技术检测GSTM1和GSTT1基因缺失,PCR-RFLP技术检测GSTP1基因单核苷酸多态(第105密码子A→G)。结果:GSTM1和GSTT1基因缺失频率在病例组和对照组差异无显著性;GSTP1A/A、A/G和G/G基因型频率分布在病例组和对照组差异无显著性;与GSTP1A/A基因型携带者相比,G/G基因型者发生直肠癌的危险性无显著升高,调整OR值为1.11(95%CI:0.77～1.60)。结论:GSTM1、GSTT1和GSTP1基因多态性与直肠癌易感性无关。

Objective：To study the genetic polymorphisms of GSTM1 ,GSTT1 and GSTP1 and the susceptibility of rectal cancer. Methods：The study was conducted with 210 cases of rectal cancer and 439 cases of normal controls. The epidemiological data were collected, and the genotypes of the GSTM1 and GSTT1 were detected by multiplex PCR. Polymorphism of GSTPI （A→G） was detected using PCR-based restriction fragment length polymorphisms （PCR-RFL P）. Results：No significant difference was found in the frequencies of the deletion genotypes among the patients and controls No significant difference was found in the frequencies of GSTPI A/A, A/G and G/G genotypes among the patients and controls. Creased risk of rectal cancer was not significanthy associated with GSTPI G/G genotypes （adjusted for age, sex and status of the smoking and alcohol drinking （OR = 1.11, 95% CI：0.77-1.60） compared with those with GSTPI A/A genotypes. Conclusion：The polymorphisms of GSTM1 ,GSTT1 and GSTP1 may not be associated with the susceptibility of rectal cancer.