哮喘患儿血清可溶性P-、L-选择素表达的变化及意义

The Serum Expression of Soluble P-selectin and L-selectin and Its Significance in Children with Asthma

目的观察可溶性P-、L-选择素在哮喘急性期、缓解期患儿和健康儿童血清中的变化,探讨其在哮喘患儿中的表达及意义.方法选取儿童哮喘急性期病例42例、缓解期病例18例及健康对照组儿童20例,应用双抗体夹心酶联免疫吸附法(ELISA)分别测定其血清可溶性P-、L-选择素浓度并进行统计分析.结果血清P-选择素浓度哮喘急性期组较缓解期组和健康儿童组明显增高(P<0.01),而哮喘缓解期组和健康儿童组相比无统计学意义(P>0.05);血清L-选择素浓度哮喘急性期组和缓解期组较健康儿童组明显升高(P<0.01),而哮喘急性期组和缓解期组相比无统计学意义(P>0.05).结论血清可溶性P-选择素水平可以反映气道炎症严重程度,L-选择素可反映儿童哮喘的气道炎症及高反应性情况.

Objective To investigate the serum expression of soluble P - selectin and L - selectin and its significance in children with asthma. Methods The serum expression of soluble P - selectin and L - selectin were detected in 42 children with acute asthma attacks, 18 children with stable asthma and 20 healthy children by using a sandwich ELISA procedure. Results The serum expression of soluble P - selectin in children with acute asthma attacks was significantly higher than those in children with stable asthma and healthy children （P 〈 0. 01 ） while no significant difference between the latter two groups （P 〉0. 05）. The serum expression of soluble L- selectin in children with acute asthma attacks and children with stable asthma was significantly higher than that in healthy children （P 〈0. 01 ） while no significant difference between the former two groups （P 〉 0. 05 ）. Gonolusions The serum expression of soluble P - selectin can reflect severity of asthma while L - selectin can reflect air passage inflammation and hyperreactivity.