结直肠癌组织中人细小病毒B19的检测

Detection of human parvovirus B19 in human colorectal cancer

目的探讨人细小病毒B19感染与结直肠癌发生的关系。方法运用原位杂交对50例石蜡包埋结直肠癌患者的肿瘤组织,癌周结直肠组织以及10例正常成人结肠组织中B19病毒进行检测。激光捕获显微切割肿瘤细胞及癌周正常肠上皮细胞,巢式PCR扩增B19DNA。结果50例结直肠癌标本中,原位杂交示B19阳性信号在肿瘤组织为78%(39/50),癌周结直肠组织为40%(20/50),正常结肠组织中5例(n=10),经统计分析肿瘤与癌周组织B19感染差异有显著性(P<0.01),正常结肠组织与癌周组织间未见统计学差异(P=1.000)。显微切割进一步证实B19病毒DNA存在于肿瘤细胞内。结论结直肠组织中人细小病毒B19感染较常见,主要存在于结直肠癌上皮细胞内,该病毒可能在结直肠癌的发生过程中起一定作用。

Purpose To investigate the possible relationship between parvovirus B19 and the pathogenesis of human colorectal cancer. Methods The parvovirus B19 DNA was examined by in situ hybridization in 50 cases of paraffin-embedded tumor tissues and matched tumor-adjacent tissues from patients with colorectal carcinoma, as well as 10 cases of normal colon as control. In addition, ten cases selected randomly were detected by laser captured microdissection followed by nested-PCR. Results In situ hybridization studies showed B19 positive signal in 39 of S0 （78%） cases and 20 of 50 （40%） of the tumor and tumor-adjacent tissues respectively. Statistical analysis indicated that the B19 positive rates in the tumors differed significantly from that in matched tissues （P 〈0. 01 ）. Five of 10 cases of normal colon had B19 signal, but no significant difference （P = 1. 000）. Furthermore, laser captured microdissection confirmed that the B19 DNA was located in the neoplastic cells of colorectal adenocarcinoma. Conclusions Parvovirus B19 infection is common in colorectum, and seems preferentially located in the neoplastic cells, which might be linked with the carcinogenesis of colon.