影响白菜游离小孢子培养关键因素分析

Isolated Microspore Culture in Brassica campestris ssp.chinensis

对影响白菜游离小孢子培养的关键因素进行分析研究。单因素研究结果表明：54个基因型之间的小孢子胚诱导率差异显著；对供体材料低温处理，在0～5d范围内小孢子胚诱导率差异不大，超过5d则明显降低；高温诱导在12～60h范围内差异不大，超过此范围则小孢子胚诱导率明显降低；NLN培养基中添加生长素（NAA）和细胞分裂素（6-BA）对小孢子胚诱导率影响不大，添加的浓度过大时小孢子胚诱导率反而降低；活性炭的有无和浓度大小对小孢子胚诱导率影响极大。通过基因型、生长素、细胞分裂素、活性炭4因素分析表明：基因型之间差异显著，活性炭不同浓度之间差异显著；基因型与活性炭不同浓度之间的互作差异显著；其它的5个一级互作、4个二级互作和1个三级互作差异均不显著。

Several factors affecting isolated microspore culture of non-heading Chinese cabbage （Brassica campestris ssp. chinensis） were studied in experiments. The results from mono-factor experiments showed that there were significant differences in the frequency of microspore-induced embryos among different genotypes. In the low temperature treatment, no significant differences were found during 0 -5 days, while the frequencies were obviously lower when the period was longer than five days. There were no significant differences in high temperature when the incubation time was within 12 -60 h, but the frequencies of microspore-induced embryos were much lower over this duration. The addition of auxins （NAA） and the cytokinins （6-BA） in NLN medium showed no significant effect on the frequencies of microspore-induced embryos, but when the concentration was excessive, the frequencies decreased. The presence and concentration of activated charcoal had a great influence on the frequency of microspore-induced embryos. Mo- reover, four-factor analysis of genotype, auxin, cytokinins and activated charcoal concentrations indicated that there were significant differences among different genotypes and activated charcoal concentrations, respectively, and also significant differences among these two interactions, while other interactions remained non-significant.