摘要
采用响应曲面法的Box-Behnken设计,研究了高静压对草莓过氧化物酶(POD)的钝化作用。试验结果表明:高静压处理可显著钝化草莓POD活性。因素(压力、温度和时间)以及因素间交互作用的分析显示,在本试验条件下压力对钝化POD起到关键作用。采用DesignExpert软件对数据进行处理,建立了高静压钝化草莓POD的响应模型,y=97.2-44.0x1-1.1x2-5.3x3-27.5x12-7.3x22-9.4x32-8.3x1x2-5.0x1x3+26.9x2x3,决定系数R2=0.9883,调整决定系数R2=0.9732。方差分析表明,模型极显著,因此该模型可用于POD钝化效果分析和预测。
The passivation effect of high hydrostatic pressure on POD （peroxidase） of strawberry was studied by response surface methodology. Experimental results showed that high hydrostatic pressure could passivate POD significantly. Based on analysis of the factors （pressure, temperature and time） and their interaction, it was found that pressure was a key factor to passivate POD under the conditions of this experiment. A model, y = 97.2 - 44. 0x1 - 1.1 x2 - 5.3x3 - 27.5x1^2 - 7.3x2^2 - 9. 4x3^2 - 8.3x1x2 - 5.0x1x3 ＋ 26.9x2x3, was established by Design Expert software. The determination coefficient of model was 0. 9883 and adjusted determination coefficient was 0. 9732. Analysis of variance implied that the model was significant. Therefore, the model could be used to analyse and predict activity passivation of POD.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期209-212,共4页
Acta Horticulturae Sinica
基金
安徽省教育厅重点科研项目(2004kj004zd)
农产品生物化工教育部重点实验室开放基金资助项目(NKF2004005)
关键词
草莓
高静压
POD
钝化
响应曲面法
Strawberry
High hydrostatic pressure
POD
Passivation
Response surface methodology
