光和糖对非洲菊花色素苷积累及CHS、DFR基因表达的影响

Anthocyanin Accumulation and CHS,DFR Gene Expression Regulated by Light and Sugar in Gerbera hybrida Ray Floret

探讨了光和糖对非洲菊(Gerbera hybrida)花序最外轮舌状花(ray floret)花瓣着色的影响。P2期花序和舌状花先在含有3%蔗糖和无蔗糖的固体培养基(8g.L-1琼脂)上进行3d暗培养,光照处理不同时间后,继续暗培养,第9天取样,采用分光光度计法测定舌状花花瓣花色素苷积累;照光后立即取样,以Northern-blot检测CHS、DFR基因表达的变化。研究结果表明,短暂光照提高花色素苷积累;光照明显诱导CHS、DFR基因表达,光照时间越长,基因表达水平也越高。暗培养7d和9d,光诱导基因表达效应明显减弱。培养基中含有蔗糖时,光强烈诱导花色素苷积累和CHS、DFR基因表达。

Effects of light and sugar on Gerbera hybrida ray floret （rf） pigmentation were investigated. Inflorescences or rfs at stage P2 were detached and incubated on the medium （8 g·L^-1 agar with 3% sucrose or free of sucrose） under 14 h light/10 h dark cycles at 23 -25℃. Cultured inflorescences and rfs were first incubated in darkness for 3 d, then exposed to transient light for a period of time followed by an incubation in the dark until harvesting for anthocyanin determination by spectrophotometer at 9th d or harvested immediately for gene expression detected by Northern-blot. The results showed that transient light exposure induced anthocyanin accumulation and the gene expression of CHS and DFR in rf petal after 3 d preculture in darkness. The longer time of light irradiation strongly enhanced the gene expression of CHS and DFR . Petals precultured in darkness for 7 d and 9 d had weak response to light in gene expression. When sucrose was present in the medium, both anthocyanin accumulation and gene expression were strongly increased under light condition.